Pig Nuclear factor-kappa B,NF-κB ELISA Kit

產(chǎn)品詳情

說(shuō)明書(shū)下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

豬核因子-κB(NF-κB)酶聯(lián)免疫試劑盒（CSB-E13448p）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿、細(xì)胞裂解物樣本中的NF-κB含量。NF - κB是重要的轉(zhuǎn)錄因子，廣泛存在于多種細(xì)胞中。在細(xì)胞的存活、增殖、炎癥反應(yīng)等生理病理過(guò)程中起到關(guān)鍵作用。研究機(jī)制上，它受多種信號(hào)通路調(diào)控，激活后能轉(zhuǎn)位至細(xì)胞核調(diào)控基因表達(dá)，其異常與腫瘤、炎癥等多種疾病相關(guān)。試劑盒檢測(cè)范圍為31.25 pg/mL-2000 pg/mL，該產(chǎn)品適用于動(dòng)物模型構(gòu)建、病原體感染機(jī)制研究、疫苗效力評(píng)估等科研領(lǐng)域本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書(shū)。
別名：

NF-kappa B,Nuclear factor-kappa B
縮寫(xiě)：

NF-κB
種屬：

Sus scrofa (Pig)
樣本類型：

serum, plasma, tissue homogenates, cell lysates
檢測(cè)范圍：

31.25 pg/mL-2000 pg/mL
靈敏度：

7.81 pg/mL
反應(yīng)時(shí)間：

1-5h
樣本體積：

50-100ul
檢測(cè)波長(zhǎng)：

450 nm
研究領(lǐng)域：

Epigenetics and Nuclear Signaling
測(cè)定原理：

quantitative
測(cè)定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of pig NF-κB in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	90
	Range %	87-95
1:2	Average %	103
	Range %	99-106
1:4	Average %	93
	Range %	87-98
1:8	Average %	91
	Range %	88-95

回收率：

The recovery of pig NF-κB spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	96	92-102
EDTA plasma (n=4)	90	86-93

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.