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  4. Pig Pancreatic triacylglycerol lipase(PNLIP) ELISA kit

Pig Pancreatic triacylglycerol lipase(PNLIP) ELISA kit

  • 中文名稱：
    豬胰腺三酰基甘油脂肪酶(PNLIP)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-EL018262PI
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥4200/￥3000
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    豬胰腺三?；视椭久?PNLIP)酶聯(lián)免疫試劑盒（CSB-EL018262PI）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿、細(xì)胞裂解物樣本中的PNLIP含量。PNLIP即胰腺脂肪酶，是消化脂肪的關(guān)鍵酶。其背景在于它對膳食脂肪的消化吸收至關(guān)重要。研究機(jī)制方面，聚焦于其催化脂肪水解的過程，了解其活性調(diào)節(jié)機(jī)制，以此為靶點開發(fā)調(diào)節(jié)脂肪消化的藥物，為肥胖、高脂血癥等治療提供思路。試劑盒檢測范圍為0.625 mU/mL-40 mU/mL，該產(chǎn)品適用于動物模型研究、營養(yǎng)學(xué)實驗中胰腺功能評估、消化酶活性動態(tài)監(jiān)測，以及藥物或化合物對脂質(zhì)代謝影響的體外分析。實驗操作兼容多種生物樣本類型，為探索胰腺生理機(jī)制、代謝性疾病發(fā)病機(jī)理等基礎(chǔ)研究提供可靠工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    PNLIPPancreatic triacylglycerol lipase ELISA Kit; PL ELISA Kit; PTL ELISA Kit; Pancreatic lipase ELISA Kit; EC 3.1.1.3 ELISA Kit
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Sus scrofa (Pig)
  • 樣本類型：
    serum, plasma, tissue homogenates, cell lysates
  • 檢測范圍：
    0.625 mU/mL-40 mU/mL
  • 靈敏度：
    0.156 mU/mL
  • 反應(yīng)時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Metabolism
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：

    Intra-assay Precision (Precision within an assay): CV%<8%

    Three samples of known concentration were tested twenty times on one plate to assess.

    Inter-assay Precision (Precision between assays): CV%<10%

    Three samples of known concentration were tested in twenty assays to assess.

  • 線性度：

    To assess the linearity of the assay, samples were spiked with high concentrations of pig PNLIP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

    　

    Sample

    Serum(n=4)

    1:1

    Average %

    94

    Range %

    86-100

    1:2

    Average %

    95

    Range %

    82-107

    1:4

    Average %

    92

    Range %

    81-103

    1:8

    Average %

    96

    Range %

    88-103

  • 回收率：

    The recovery of pig PNLIP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range

    Serum (n=5)

    95

    87-106

    EDTA plasma (n=4)

    97

    89-112

  • 標(biāo)準(zhǔn)曲線：

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

    mU/ml

    OD1

    OD2

    Average

    Corrected

    40

    2.052

    2.137

    2.095

    2.007

    20

    1.639

    1.658

    1.649

    1.561

    10

    1.110

    1.134

    1.122

    1.034

    5

    0.753

    0.711

    0.732

    0.644

    2.5

    0.405

    0.392

    0.399

    0.311

    1.25

    0.312

    0.303

    0.308

    0.220

    0.625

    0.189

    0.181

    0.185

    0.097

    0

    0.086

    0.090

    0.088

    　

  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評價

靶點詳情

  • 功能：
    Plays an important role in fat metabolism. It preferentially splits the esters of long-chain fatty acids at positions 1 and 3, producing mainly 2-monoacylglycerol and free fatty acids, and shows considerably higher activity against insoluble emulsified substrates than against soluble ones.
  • 亞細(xì)胞定位：
    Secreted.
  • 蛋白家族：
    AB hydrolase superfamily, Lipase family
  • 數(shù)據(jù)庫鏈接：