Pig Progonadoliberin-2(GNRH2) ELISA kit
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中文名稱:豬促性腺素釋放素原-2(GNRH2)酶聯(lián)免疫試劑盒
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貨號(hào):CSB-EL009636PI
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規(guī)格:96T/48T
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價(jià)格:¥4200/¥3000
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其他:
產(chǎn)品詳情
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產(chǎn)品描述:豬促性腺素釋放素原-2(GNRH2)酶聯(lián)免疫試劑盒(CSB-EL009636PI)為競爭法ELISA試劑盒,定量檢測血清、血漿、組織勻漿樣本中的GNRH2含量。GNRH2即促性腺激素釋放激素2,它在生殖內(nèi)分泌系統(tǒng)中扮演重要角色。研究認(rèn)為它通過與特異性受體結(jié)合,調(diào)控垂體促性腺激素的合成與分泌,進(jìn)而影響生殖功能。目前對(duì)其作用機(jī)制的深入研究,有望為生殖疾病的診治提供新方向。試劑盒檢測范圍為40 pg/mL-8000 pg/mL,廣泛應(yīng)用于動(dòng)物生殖生理機(jī)制研究,包括繁殖性能評(píng)估、生殖內(nèi)分泌調(diào)控網(wǎng)絡(luò)解析以及激素相關(guān)疾病模型的分子機(jī)制探索,也可為畜牧業(yè)中繁殖管理策略的優(yōu)化提供數(shù)據(jù)支持。本品僅用于科研,不用于臨床診斷,產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
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別名:GnRH-II, LH-RHII, OTTHUMP00000030076|OTTHUMP00000030077|luliberin II|luteinizing hormone-releasing hormone II|progonadoliberin II
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縮寫:GNRH2
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種屬:Sus scrofa (Pig)
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樣本類型:serum, plasma, tissue homogenates
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檢測范圍:40 pg/mL-8000 pg/mL
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靈敏度:20 pg/mL
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反應(yīng)時(shí)間:1-5h
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樣本體積:50-100ul
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檢測波長:450 nm
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研究領(lǐng)域:Signal Transduction
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測定原理:quantitative
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測定方法:Competitive
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精密度:
Intra-assay Precision (Precision within an assay): CV%<15% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<15% Three samples of known concentration were tested in twenty assays to assess. -
線性度:
To assess the linearity of the assay, samples were spiked with high concentrations of pig GNRH2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:1 Average % 87 Range % 81-92 1:2 Average % 96 Range % 90-105 1:4 Average % 97 Range % 92-105 1:8 Average % 94 Range % 88-103 -
回收率:
The recovery of pig GNRH2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 95 90-99 EDTA plasma (n=4) 97 89-105 -
標(biāo)準(zhǔn)曲線:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. pg/ml OD1 OD2 Average 8000 0.186 0.189 0.188 2000 0.321 0.325 0.323 640 0.520 0.518 0.519 160 0.811 0.807 0.809 40 1.321 1.326 1.324 0 1.967 1.988 1.978 -
數(shù)據(jù)處理:
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貨期:3-5 working days
引用文獻(xiàn)
- LH-Independent Testosterone Secretion Is Mediated by the Interaction Between GNRH2 and Its Receptor Within Porcine Testes Desaulniers AT. et al,Biol Reprod.,2015
- Renal Tubular Damage May Contribute More to Acute Hyperglycemia Induced Kidney Injury in Non-diabetic Conscious Rats Jing-Yu Wang et al,ScienceDirect,2015
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