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  4. Pig Soluble protein-100B,S-100B ELISA Kit

Pig Soluble protein-100B,S-100B ELISA Kit

  • 中文名稱：
    豬S100B蛋白(S-100B)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-E14063p
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥4200/￥3000
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    豬S100B蛋白(S-100B)酶聯(lián)免疫試劑盒（CSB-E14063p）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的S-100B含量。S-100B是一種鈣結(jié)合蛋白，主要存在于神經(jīng)膠質(zhì)細(xì)胞中。其背景上與神經(jīng)系統(tǒng)損傷、疾病相關(guān)。研究機(jī)制方面，它可作為生物標(biāo)志物反映中樞神經(jīng)系統(tǒng)損傷程度，還參與細(xì)胞增殖、分化等過程，在神經(jīng)病理生理活動(dòng)中發(fā)揮重要作用。試劑盒檢測范圍為78 pg/mL-5000 pg/mL，本試劑盒通過高特異性抗體對目標(biāo)蛋白進(jìn)行捕獲與檢測，可穩(wěn)定應(yīng)用于神經(jīng)損傷模型研究、疾病機(jī)制探索或藥物干預(yù)效果評價(jià)等科研場景，為分析豬體內(nèi)S100B蛋白的動(dòng)態(tài)變化提供可靠工具。樣本兼容性強(qiáng)，支持直接檢測未經(jīng)稀釋的體液樣本或經(jīng)適當(dāng)處理的組織勻漿，操作流程標(biāo)準(zhǔn)化，滿足實(shí)驗(yàn)室對神經(jīng)生物學(xué)、獸醫(yī)學(xué)或比較醫(yī)學(xué)等領(lǐng)域的研究需求。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 縮寫：
    S-100B
  • Uniprot No.：
  • 種屬：
    Sus scrofa (Pig)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    78 pg/mL-5000 pg/mL
  • 靈敏度：
    19.5 pg/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Signal Transduction
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of pig S-100B in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %95
    Range %91-100
    1:2Average %93
    Range %85-101
    1:4Average %86
    Range %80-98
    1:8Average %94
    Range %92-105
  • 回收率：
    The recovery of pig S-100B spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9583-102
    EDTA plasma (n=4)9592-100
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    50002.025 2.067 2.046 1.887
    25001.574 1.524 1.549 1.390
    12501.183 1.169 1.176 1.017
    6250.821 0.812 0.817 0.658
    3120.567 0.534 0.551 0.392
    1560.354 0.373 0.364 0.205
    780.250 0.262 0.256 0.097
    00.164 0.153 0.159 　
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評價(jià)