Pig Thyroxine(T4) ELISA Kit

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產(chǎn)品詳情

產(chǎn)品描述：

豬甲狀腺素(T4)酶聯(lián)免疫試劑盒（CSB-E13718p）為競爭法ELISA試劑盒，定量檢測血清、血漿樣本中的T4含量。試劑盒檢測范圍為20 ng/mL-320 ng/mL，靈敏度為20 ng/mL。適用于農(nóng)業(yè)科研中豬的生理周期監(jiān)測、營養(yǎng)代謝研究、繁殖性能評估以及環(huán)境應(yīng)激對內(nèi)分泌系統(tǒng)影響的實(shí)驗(yàn)分析，為動物模型構(gòu)建和基礎(chǔ)醫(yī)學(xué)研究提供可靠數(shù)據(jù)支持。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
縮寫：

T4
種屬：

Sus scrofa (Pig)
樣本類型：

serum, plasma
檢測范圍：

20 ng/mL-320 ng/mL
靈敏度：

20 ng/mL
反應(yīng)時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Signal Transduction
測定原理：

quantitative
測定方法：

Competitive

精密度：

Intra-assay Precision (Precision within an assay): CV%<15%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<15%
Three samples of known concentration were tested in twenty assays to assess.

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of pig T4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	88
	Range %	82-100
1:2	Average %	89
	Range %	83-98
1:4	Average %	95
	Range %	89-100
1:8	Average %	95
	Range %	90-100

回收率：

The recovery of pig T4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	90-99
EDTA plasma (n=4)	91	86-99

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

最新研究進(jìn)展：

一些最新的研究顯示T4蛋白參與了神經(jīng)遞質(zhì)的調(diào)節(jié)和心血管系統(tǒng)的正常功能，未來的研究將更加關(guān)注T4蛋白在這些領(lǐng)域中的作用。