Pig Tumor necrosis factor (TNF/TNFA/TNFSF2) ELISA kit
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中文名稱:豬腫瘤壞死因子alpha(TNF/TNFA/TNFSF2)酶聯(lián)免疫試劑盒
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貨號:CSB-E16980p-IS
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規(guī)格:96T/48T
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價(jià)格:¥3600/¥2500
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其他:
產(chǎn)品詳情
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產(chǎn)品描述:豬腫瘤壞死因子alpha(TNF/TNFA/TNFSF2)酶聯(lián)免疫試劑盒(CSB-E16980p-IS)為雙抗夾心法ELISA試劑盒,定量檢測血清、血漿、組織培養(yǎng)上清液、組織勻漿樣本中的TNF含量。TNF(腫瘤壞死因子)是一種多功能細(xì)胞因子,在機(jī)體免疫調(diào)節(jié)、炎癥反應(yīng)等過程中起關(guān)鍵作用。其背景在于它是機(jī)體應(yīng)對感染和組織損傷的重要介質(zhì)。研究機(jī)制主要聚焦于它與受體結(jié)合后激活多條信號通路,進(jìn)而調(diào)控細(xì)胞的存活、凋亡、炎癥因子釋放等。試劑盒檢測范圍為23.5 pg/mL-1500 pg/mL,靈敏度為11.75 pg/mL。廣泛應(yīng)用于動物模型研究、體外細(xì)胞實(shí)驗(yàn)中的炎癥因子動態(tài)監(jiān)測、免疫調(diào)節(jié)藥物篩選及疫苗效力評估等科研場景,為探索豬源性疾病機(jī)制、評估生物制劑效果提供可靠工具。本品僅用于科研,不用于臨床診斷,產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
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別名:TNF; TNFA; TNFSF2; Tumor necrosis factor; Cachectin; TNF-alpha; Tumor necrosis factor ligand superfamily member 2; TNF-a
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縮寫:
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Uniprot No.:
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種屬:Sus scrofa (Pig)
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樣本類型:serum, plasma, cell culture supernates, tissue homogenates
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檢測范圍:23.5 pg/mL-1500 pg/mL
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靈敏度:11.75 pg/mL
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反應(yīng)時(shí)間:1-5h
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樣本體積:50-100ul
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檢測波長:450 nm
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研究領(lǐng)域:Immunology
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測定原理:quantitative
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測定方法:Sandwich
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精密度:
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
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線性度:
To assess the linearity of the assay, samples were spiked with high concentrations of pig TNF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
Sample
Serum(n=4)
1:1
Average %
90
Range %
85-95
1:2
Average %
98
Range %
93-103
1:4
Average %
95
Range %
90-102
1:8
Average %
93
Range %
87-98
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回收率:
The recovery of pig TNF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type
Average % Recovery
Range
Serum (n=5)
93
86-100
EDTA plasma (n=4)
87
82-94
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標(biāo)準(zhǔn)曲線:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml
OD1
OD2
Average
Corrected
1500
2.909
2.834
2.872
2.699
750
1.806
1.697
1.752
1.579
375
0.990
1.028
1.009
0.836
187.5
0.567
0.589
0.578
0.405
94
0.344
0.321
0.332
0.159
47
0.277
0.283
0.280
0.107
23.5
0.228
0.214
0.221
0.048
0
0.176
0.170
0.173
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數(shù)據(jù)處理:
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貨期:3-5 working days
引用文獻(xiàn)
- Hydroxy-Selenomethionine Supplementation During Gestation and Lactation Improve Reproduction of Sows by Enhancing the Antioxidant Capacity and Immunity Under Heat Stress Conditions J Wang, H Sun, Z Peng, SQ Wang, YQ Yan, WC Luo,Antioxidants,2025
相關(guān)產(chǎn)品
靶點(diǎn)詳情
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功能:Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation. Induces insulin resistance in adipocytes via inhibition of insulin-induced IRS1 tyrosine phosphorylation and insulin-induced glucose uptake. Induces GKAP42 protein degradation in adipocytes which is partially responsible for TNF-induced insulin resistance. Plays a role in angiogenesis by inducing VEGF production synergistically with IL1B and IL6.; The TNF intracellular domain (ICD) form induces IL12 production in dendritic cells.
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基因功能參考文獻(xiàn):
- these findings provide the direct evidence that ADAM17 cleaves porcine TNFalpha, which represents a new view for identifying potential therapeutic targets in anti-porcine reproductive and respiratory syndrome virus therapy PMID: 26724939
- TMZ pretreatment effectively reduced the myocardial damage caused by CME via inhibiting the PDCD4/NF-kappaB/ TNF-alpha pathway in cardiomyocytes. PMID: 28683436
- TNF-alpha, secreted from activated Monocytes, mediates the downregulation of OTX2 and essential retinal pigment epithelium genes. PMID: 27660103
- TNF-alpha induced MMP-13 expression by condylar cells might be involved in the degradation of the juvenile condyle. PMID: 27618228
- TNF-alpha was able to promote theca interna cell proliferation. Our results suggest that TNF-alpha might play a role in hyperandrogenism, cortex thickness, and the increased ovary volume observed in polycystic ovaries. PMID: 27125399
- This study showed that the -791(C-->T) mutation of the TNF-alpha gene could be considered an important potential genetic marker of enterotoxigenic Escherichia coli F18 resistance. PMID: 27408333
- Both Nsp1beta and Nsp11 of porcine reproductive and respiratory syndrome virus were demonstrated to be responsible for the inhibitory effect on TNF-alpha production in pulmonary alveolar macrophages. PMID: 25708177
- Study presents evidence demonstrating a single species of exotoxin ApxI, derived from A. pleuropneumoniae serotype 10, induces the expression and production of proinflammatory cytokines IL-1beta, IL-8 and TNF-alpha in porcine alveolar macrophages. PMID: 21314908
- the role of miR-181a in adipocyte differentiation by regulation of TNF-alpha PMID: 24098322
- The NOS inhibitor, L-NMMA, significantly suppressed the combined effects of HT and CORM-2 on TNFalpha-triggered NFkappaBp65 phosphorylation as well as decreased cell viability. PMID: 24066302
- High-volume hemofiltration improves hemodynamics and heart dysfunction in septic shock pigs, which may be attributed to reduction of TNF-alpha in myocardium but not in circulation. PMID: 22994292
- Basal lipogenesis was not affected by tumor necrosis factor alpha (TNFalpha) treatment; however, insulin stimulated lipogenesis was reduced by TNFalpha. Interleukin 6 and TNFalpha gene expression were acutely (2-4 h) stimulated by exogenous TNFalpha treatment. PMID: 23090779
- In swine, IL-8, TNF-ALPHA, INOS AND MIP-1BETA were increased during mechanical ventilation in a time-related fashion. PMID: 23102097
- These data demonstrate that CRF triggers increases in intestinal paracellular permeability via mast cell dependent release of TNF-alpha and proteases. PMID: 22768175
- We identified critical amino acid residues in PRRSV Nsp1alpha and Nsp1beta that are important for TNF-alpha down-regulation and attenuation in vivo. PMID: 22699004
- Porcine reproductive and respiratory syndrome virus infection impaired TNF-alpha production by inhibiting ERK signaling. pathway. PMID: 22497732
- Data show that all five molecules, BNP, ICAM-1, TNF-alpha, VCAM-1 and IL-6, quickly and reliably signaled adverse interactions. PMID: 21471537
- These results suggest that trans-10, cis-12-conjugated linoleic acid can modulate TNF-alpha production and NF-kappa B expression by a PPARgamma-dependent pathway in porcine peripheral blood mononuclear cells. PMID: 21205430
- Retinal ischemia results in increased expression of TNF-alpha and its receptors (TNF-R1 and TNF-R2). PMID: 21152396
- increased plasma levels during recovery from cardiac arrest are associated with left ventricle dysfunction PMID: 19642909
- Viral non-structural protein 1 suppressed host TNF-alpha promoter by inhibiting NF-kappaB and Sp1 promoter binding activity. PMID: 20701940
- Exposure of porcine oocytes to an elevated TNF-alpha concentration clearly caused a reduction in their maturation from GV stage to MII stage and increased the proportion of oocytes with abnormal chromosome alignment and cytoskeleton structure PMID: 19324350
- Survival in the setting of severe, acute trauma/hemorrhagic shock appears to be associated with an immediate increase in serum TNF-alpha. PMID: 20027315
- These results suggest that TNF-alpha and IL-10, but not IL-6, are involved in the late reparatory phases of the experimental disk lesion. PMID: 19605905
- Repression of TNF-alpha associated with moderate hypothermia during experimental cardiac surgery is due to inhibition of the mitogen-activated protein kinase p38/activating protein-1 pathway and not to inhibition of NF-kappa B. PMID: 16606437
- Stenting of coronary arteries was associated with an eight-fold enhancement in TNFalpha expression in injured arterial tissue. PMID: 16857205
- The effect of EGF on pro-inflammatory cytokines, tumor necrosis factor-alpha (TNF-alpha) and cyclooxygenase-2 (COX-2), levels during wound healing in swine is reported. PMID: 17098266
- In coronary microembolization, TNF-alpha is responsible for both progressive contractile dysfunction and delayed protection against infarction. PMID: 17170366
- The data indicate that the development of clinical symptoms of coliform mastitis in the sow is associated with a locally increased proinflammatory cytokine production in response to intramammary Escherichia coli infection. PMID: 17903420
- the ERK/GEF-H1/Rho/Rho kinase/phospho-MLC pathway is the mechanism mediating TNF-alpha-induced elevation of tubular epithelial permeability, which in turn might contribute to kidney injury PMID: 19261619
- Data show that cysteine significantly reduced the expression of pro-inflammatory cytokines, including TNF-alpha, IL-6, IL-12p40, IL-1beta, and resulted in increased expression of the apoptosis initiator caspase-8 and bcl2L1. PMID: 19520150
- TNF-alpha inhibits adipogenesis through stabilization of beta-catenin protein in porcine preadipocytes. PMID: 19558791
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亞細(xì)胞定位:Cell membrane; Single-pass type II membrane protein.; [Tumor necrosis factor, membrane form]: Membrane; Single-pass type II membrane protein.; [Tumor necrosis factor, soluble form]: Secreted.; [C-domain 1]: Secreted.; [C-domain 2]: Secreted.
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蛋白家族:Tumor necrosis factor family
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數(shù)據(jù)庫鏈接:
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