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  4. Pig Vascular cell adhesion molecule 1,VCAM-1 ELISA Kit

Pig Vascular cell adhesion molecule 1,VCAM-1 ELISA Kit

  • 中文名稱：
    豬血管內(nèi)皮細(xì)胞粘附分子1(VCAM-1/CD106)酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-E06825p
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥4200/￥3000
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    豬血管內(nèi)皮細(xì)胞粘附分子1(VCAM-1/CD106)酶聯(lián)免疫試劑盒（CSB-E06825p）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的VCAM1含量。VCAM1（血管細(xì)胞粘附分子1）是一種細(xì)胞表面糖蛋白，屬于免疫球蛋白超家族，主要表達(dá)于活化的內(nèi)皮細(xì)胞表面。它通過介導(dǎo)白細(xì)胞與內(nèi)皮細(xì)胞的粘附，在免疫反應(yīng)和炎癥過程中發(fā)揮重要作用。研究顯示，VCAM1在多種疾病中扮演關(guān)鍵角色，如動(dòng)脈粥樣硬化、炎癥性腸病和癌癥，其表達(dá)水平與疾病進(jìn)展密切相關(guān)。試劑盒檢測(cè)范圍為15.6ng/mL-1000ng/mL，適用于研究豬心血管疾病模型中的內(nèi)皮細(xì)胞活化機(jī)制、炎癥性疾病病理進(jìn)程評(píng)估，以及免疫調(diào)節(jié)藥物對(duì)血管內(nèi)皮功能影響的體外實(shí)驗(yàn)，為探索VCAM - 1在血管相關(guān)疾病中的作用提供可靠工具本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 縮寫：
    VCAM1/CD106
  • 種屬：
    Sus scrofa (Pig)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測(cè)范圍：
    15.6ng/mL-1000ng/mL
  • 靈敏度：
    3.9 ng/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測(cè)波長(zhǎng)：
    450 nm
  • 研究領(lǐng)域：
    Signal Transduction
  • 測(cè)定原理：
    quantitative
  • 測(cè)定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of pig VCAM-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:1Average %92
    Range %85-94
    1:2Average %94
    Range %87-98
    1:4Average %103
    Range %97-107
    1:8Average %101
    Range %95-104
  • 回收率：
    The recovery of pig VCAM-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9490-98
    EDTA plasma (n=4)8983-94
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2AverageCorrected
    10002.335 2.178 2.257 2.100
    5001.574 1.475 1.525 1.368
    2501.007 0.982 0.995 0.838
    1250.591 0.586 0.589 0.432
    62.50.386 0.394 0.390 0.233
    31.20.278 0.282 0.280 0.123
    15.60.228 0.225 0.227 0.070
    00.158 0.156 0.157
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

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