Pig adiponectin,ADP ELISA Kit

產(chǎn)品詳情

文獻引用 (8) 評價 (1) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

豬脂聯(lián)素(ADP)酶聯(lián)免疫試劑盒（CSB-E06839p）為競爭法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的ADP含量。ADP是一種重要的靶點。它是生物體內(nèi)能量代謝的關(guān)鍵分子，參與細胞內(nèi)多種生理過程。在研究機制上，ADP能與特定受體結(jié)合，觸發(fā)細胞內(nèi)信號傳導(dǎo)通路，影響細胞功能。對ADP相關(guān)機制的研究，有助于深入了解疾病發(fā)生發(fā)展，為藥物研發(fā)提供方向。試劑盒檢測范圍為1.875 μg/mL-30 μg/mL，特別適用于動物實驗中對豬模型代謝機制、營養(yǎng)干預(yù)效果或肥胖相關(guān)病理的基礎(chǔ)研究，可支持不同生物樣本中ADP的動態(tài)監(jiān)測，為畜牧營養(yǎng)學(xué)、比較醫(yī)學(xué)等領(lǐng)域的分子機制探索提供有效工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
縮寫：

ADP
Uniprot No.：

Q6PP07
種屬：

Sus scrofa (Pig)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

1.875 μg/mL-30 μg/mL
靈敏度：

1.875 μg/mL
反應(yīng)時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Cardiovascular
測定原理：

quantitative
測定方法：

Competitive

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of pig ADP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	90
	Range %	86-94
1:2	Average %	96
	Range %	92-100
1:4	Average %	83
	Range %	80-86
1:8	Average %	91
	Range %	83-98

回收率：

The recovery of pig ADP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	87-99
EDTA plasma (n=4)	93	91-96