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  4. Pig apolipoprotein A1 (Apo-A1) ELISA Kit

Pig apolipoprotein A1 (Apo-A1) ELISA Kit

  • 中文名稱：
    豬載脂蛋白A1(Apo-A1)酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-E06834p
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥4200/￥3000
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    豬載脂蛋白A1(Apo-A1)酶聯(lián)免疫試劑盒（CSB-E06834p）為競(jìng)爭(zhēng)法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的APOA1含量。APOA1是一種位于細(xì)胞膜外的分泌蛋白，是高密度脂蛋白（HDL）的主要結(jié)構(gòu)載脂蛋白，在逆向膽固醇轉(zhuǎn)運(yùn)途徑中起關(guān)鍵作用。其與細(xì)胞表面受體SR-BI相互作用，促進(jìn)膽固醇從組織到肝臟的逆向運(yùn)輸，具有抗炎、抗動(dòng)脈粥樣硬化等作用。研究顯示，APOA1與病毒感染、動(dòng)脈粥樣硬化、糖尿病、神經(jīng)系統(tǒng)疾病、腫瘤等疾病有關(guān)。試劑盒檢測(cè)范圍為80 μg/mL-2000 μg/mL，適用于豬脂代謝機(jī)制研究、心血管疾病動(dòng)物模型評(píng)估、營(yíng)養(yǎng)干預(yù)對(duì)脂蛋白代謝的影響分析等科研領(lǐng)域，為豬相關(guān)代謝性疾病的基礎(chǔ)研究提供可靠工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說(shuō)明書。
  • 別名：
    APOA1Apolipoprotein A-I ELISA Kit; Apo-AI ELISA Kit; ApoA-I ELISA Kit; Apolipoprotein A1) [Cleaved into: Proapolipoprotein A-I ELISA Kit; ProapoA-I); Truncated apolipoprotein A-I] ELISA Kit
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Sus scrofa (Pig)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測(cè)范圍：
    80 μg/mL-2000 μg/mL
  • 靈敏度：
    50 μg/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測(cè)波長(zhǎng)：
    450 nm
  • 研究領(lǐng)域：
    Cardiovascular
  • 測(cè)定原理：
    quantitative
  • 測(cè)定方法：
    Competitive
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<15%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<15%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of pig Apo-A1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:1Average %94
    Range %89-103
    1:2Average %90
    Range %85-103
    1:4Average %93
    Range %88-100
    1:8Average %89
    Range %82-97
  • 回收率：
    The recovery of pig Apo-A1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9996-104
    EDTA plasma (n=4)9086-95
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    μg/mlOD1OD2Average
    20000.342 0.348 0.345
    8000.533 0.483 0.508
    4800.682 0.657 0.670
    2001.021 0.987 1.004
    801.267 1.325 1.296
    01.903 1.954 1.929
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    Participates in the reverse transport of cholesterol from tissues to the liver for excretion by promoting cholesterol efflux from tissues and by acting as a cofactor for the lecithin cholesterol acyltransferase (LCAT). As part of the SPAP complex, activates spermatozoa motility.
  • 基因功能參考文獻(xiàn)：
    1. Alterations in the Apo A-I pattern is a good indicator of the presence and severity of infectious diseases in the pig.Lower overall amounts of Apo A-I were observed in Salmonella typhimurium and Escherichia coli infections. PMID: 25377528
    2. blood level decreases during experimentally induced acute-phase processes in pigs PMID: 15845530
    3. down-regulation of apolipoprotein A-I and A-IV messages in the liver may be mediated by interleukin 6 and tumor necrosis factor-alpha PMID: 15878672
  • 亞細(xì)胞定位：
    Secreted.
  • 蛋白家族：
    Apolipoprotein A1/A4/E family
  • 組織特異性：
    Major protein of plasma HDL, also found in chylomicrons. Synthesized predominantly in the intestine and the liver.
  • 數(shù)據(jù)庫(kù)鏈接：

    KEGG: ssc:397691

    STRING: 9823.ENSSSCP00000015994

    UniGene: Ssc.807