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  4. Pig basic fibroblast growth factor,bFGF ELISA Kit

Pig basic fibroblast growth factor,bFGF ELISA Kit

  • 中文名稱：
    豬堿性成纖維細胞生長因子(bFGF)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-E12841p
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥4200/￥3000
  • 其他：

產品詳情

  • 產品描述：
    豬堿性成纖維細胞生長因子(bFGF)酶聯(lián)免疫試劑盒（CSB-E12841p）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的FGF2含量。FGF2即成纖維細胞生長因子2，是重要的促血管生成因子。它參與多種生理過程如胚胎發(fā)育、組織修復等。研究機制上，F(xiàn)GF2能與細胞膜上受體結合，激活下游信號通路，調控細胞的增殖、分化、遷移等，在腫瘤血管生成等疾病中作用受關注。試劑盒檢測范圍為4.7 pg/mL-300 pg/mL，本試劑盒適用于體外研究，如探究豬模型中創(chuàng)傷愈合機制、干細胞培養(yǎng)條件優(yōu)化或疾病相關生長因子通路分析，為心血管疾病、再生醫(yī)學及腫瘤生物學等基礎研究提供可靠的實驗工具本品僅用于科研，不用于臨床診斷，產品具體參數(shù)及操作步驟詳見產品說明書。
  • 別名：
    FGF2Fibroblast growth factor 2 ELISA Kit; FGF-2 ELISA Kit; Basic fibroblast growth factor ELISA Kit; bFGF ELISA Kit; Heparin-binding growth factor 2 ELISA Kit; HBGF-2 ELISA Kit; Prostatic growth factor ELISA Kit; Fragments ELISA Kit
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Sus scrofa (Pig)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    4.7 pg/mL-300 pg/mL
  • 靈敏度：
    1.2 pg/mL
  • 反應時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領域：
    Cardiovascular
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of pig bFGF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:1 Average % 88  
    Range % 80-100  
    1:2 Average % 97  
    Range % 91-105  
    1:4 Average % 94  
    Range % 90-110  
    1:8 Average % 95  
    Range % 82-105  
  • 回收率：
    The recovery of pig bFGF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 95 85-104  
    EDTA plasma (n=4) 98 92-106  
                 
                 
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected  
    300 2.175 2.157 2.166 1.966  
    150 1.582 1.612 1.597 1.397  
    75 1.202 1.134 1.168 0.968  
    37.5 0.628 0.603 0.616 0.416  
    18.75 0.377 0.362 0.370 0.170  
    9.4 0.296 0.301 0.299 0.099  
    4.7 0.265 0.256 0.261 0.061  
    0 0.196 0.204 0.200 　  
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產品評價

靶點詳情

  • 功能：
    Acts as a ligand for FGFR1, FGFR2, FGFR3 and FGFR4. Also acts as an integrin ligand which is required for FGF2 signaling. Binds to integrin ITGAV:ITGB3. Plays an important role in the regulation of cell survival, cell division, cell differentiation and cell migration. Functions as a potent mitogen in vitro. Can induce angiogenesis. Mediates phosphorylation of ERK1/2 and thereby promotes retinal lens fiber differentiation.
  • 亞細胞定位：
    Secreted. Nucleus.
  • 蛋白家族：
    Heparin-binding growth factors family
  • 數(shù)據(jù)庫鏈接：