Pig cardiac troponin Ⅰ,cTn-ⅠELISA Kit

產品詳情

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說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產品描述：

豬心肌肌鈣蛋白Ⅰ(cTn-Ⅰ)酶聯免疫試劑盒（CSB-E11187p）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的cTn-I含量。cTn-I（心肌肌鈣蛋白I）是一種心肌細胞特有的調節(jié)蛋白，在心肌損傷時釋放進入血液，成為診斷心肌損傷的重要標志物。其研究機制涉及心肌損傷時cTn-I的釋放和檢測，對于早期診斷心肌梗死等心血管疾病具有重要意義。試劑盒檢測范圍為15.6 pg/mL-1000 pg/mL，適用于科研領域中心肌損傷相關機制研究；適用于動物模型中心臟疾病研究、心血管藥物開發(fā)中的藥效評估，以及體外實驗中心肌細胞損傷模型的生物標志物檢測，為科研人員提供穩(wěn)定可靠的心肌損傷動態(tài)監(jiān)測工具。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
縮寫：

cTnⅠ
Uniprot No.：

I3LLI0
種屬：

Sus scrofa (Pig)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

15.6 pg/mL-1000 pg/mL
靈敏度：

3.9 pg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Cardiovascular
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of pig cTn-Ⅰ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	94
	Range %	89-102
1:2	Average %	89
	Range %	85-99
1:4	Average %	95
	Range %	90-100
1:8	Average %	93
	Range %	83-99

回收率：

The recovery of pig cTn-Ⅰ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	97	90-101
EDTA plasma (n=4)	95	86-106

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.