Pig coagulation factor X (F10) ELISA kit

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產(chǎn)品詳情

產(chǎn)品描述：

豬凝血因子X (F10)酶聯(lián)免疫試劑盒（CSB-EL007915PI）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的F10含量。F10即凝血因子X，其在凝血級(jí)聯(lián)反應(yīng)中至關(guān)重要，激活后能促使凝血酶原轉(zhuǎn)變?yōu)槟?，推?dòng)纖維蛋白原形成纖維蛋白，完成凝血過程。針對(duì)F10的研究聚焦于開發(fā)新型抗凝藥物，抑制其活性來防治血栓性疾病。試劑盒檢測(cè)范圍為0.312 μg/mL-20 μg/mL，該產(chǎn)品適用于動(dòng)物疾病模型研究（如凝血功能障礙、肝臟疾?。?、營(yíng)養(yǎng)代謝實(shí)驗(yàn)或藥物毒理學(xué)評(píng)估等科研領(lǐng)域，為探究F10在豬凝血機(jī)制、止血調(diào)控及相關(guān)病理過程中的作用提供可靠工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
縮寫：

F10
Uniprot No.：

F1RN41
種屬：

Sus scrofa (Pig)
樣本類型：

serum, plasma, tissue homogenates
檢測(cè)范圍：

0.027 μg/mL-20 μg/mL
靈敏度：

0.027 μg/mL
反應(yīng)時(shí)間：

1-5h
樣本體積：

50-100ul
檢測(cè)波長(zhǎng)：

450 nm
研究領(lǐng)域：

Blood Coagulation
測(cè)定原理：

quantitative
測(cè)定方法：

Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of pig F10 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:5	Average %	93
	Range %	87-105
1:10	Average %	96
	Range %	90-110
1:20	Average %	92
	Range %	89-99
1:40	Average %	95
	Range %	83-100

回收率：

The recovery of pig F10 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	88-106
EDTA plasma (n=4)	96	89-102

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.