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  4. Pig endothelin 1,ET-1 ELISA Kit

Pig endothelin 1,ET-1 ELISA Kit

  • 中文名稱：
    豬內(nèi)皮素1(ET-1)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-E06806p
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥4200/￥3000
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    豬內(nèi)皮素1(ET-1)酶聯(lián)免疫試劑盒（CSB-E06806p）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的EDN1含量。EDN1即內(nèi)皮素 -1 ，是一種具有強烈而持久血管收縮作用的肽類物質(zhì)。在生理上，它參與血壓調(diào)節(jié)等過程。研究機制方面，聚焦于其與心血管、呼吸系統(tǒng)等疾病關(guān)聯(lián)，如參與肺動脈高壓發(fā)展，通過作用于受體調(diào)節(jié)血管張力等。試劑盒檢測范圍為3.12 pg/mL-200pg/mL，該產(chǎn)品適用于科研場景中評估豬心血管疾病模型、藥物干預(yù)效果或組織微環(huán)境中ET - 1的動態(tài)變化，其寬檢測范圍和高靈敏度可精準捕捉低豐度樣本的濃度差異，為心血管機制研究及藥理實驗提供可靠工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    EDN1 ELISA Kit; Endothelin-1 ELISA Kit; ET-1 ELISA Kit; Preproendothelin-1 ELISA Kit; PPET1) [Cleaved into: Big endothelin-1] ELISA Kit
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Sus scrofa (Pig)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    3.12 pg/mL-200pg/mL
  • 靈敏度：
    0.78 pg/mL
  • 反應(yīng)時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Cancer
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of pig ET-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %100
    Range %94-105
    1:2Average %97
    Range %92-101
    1:4Average %89
    Range %85-93
    1:8Average %95
    Range %91-101
  • 回收率：
    The recovery of pig ET-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9085-94
    EDTA plasma (n=4)8480-89
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    2002.401 2.449 2.425 2.354
    1001.689 1.661 1.675 1.604
    500.925 0.912 0.919 0.848
    250.491 0.459 0.475 0.404
    12.50.302 0.286 0.294 0.223
    6.250.221 0.213 0.217 0.146
    3.120.158 0.154 0.156 0.085
    00.068 0.074 0.071 　
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評價

靶點詳情

  • 功能：
    Endothelins are endothelium-derived vasoconstrictor peptides. Probable ligand for G-protein coupled receptors EDNRA and EDNRB which activates PTK2B, BCAR1, BCAR3 and, GTPases RAP1 and RHOA cascade in glomerular mesangial cells.
  • 基因功能參考文獻：
    1. In conclusion, endothelial vWF knockdown prevented angiotensin II-induced ET-1 upregulation through attenuation of NOX-mediated O2- production. PMID: 27443965
    2. sub-vasomotor concentration of ET-1 leads to vascular dysfunction by impairing endothelium-dependent NO-mediated dilation via p38 kinase-mediated production of superoxide from NADPH oxidase following ETA receptor activation PMID: 26211713
    3. ET1 was lowest in kidneys removed from live pigs, greater in kidneys from pigs with brain death, and greatest in kidneys from pigs with cardiac arrest. PMID: 25380860
    4. ET-1 contributes to formation of oedema during experimental sepsis by a novel mechanism involving increased HBP release from neutrophils. PMID: 24611481
    5. Endothelin-1 expression is upregulated following therapeutic hypothermia after cardiac arrest. PMID: 23717659
    6. Our experiment with isolated porcine ophthalmic ciliary arteries revealed a refractoriness phase to ET after an acute stimulation with ET. This refractoriness was transient and disappeared after 4 h. PMID: 23327561
    7. ET-1 produces contraction of the urinary bladder neck muscles via muscular ET(A) receptors coupled to extracellular Ca(2+) entry via VOC (L-type) and non-VOC channels. PMID: 21953705
    8. urinary biomarker to determine kidney graft injury PMID: 22221604
    9. Endothelin-1 elevation is not only a conserved phenomenon in a pig traumatic brain injury (TBI) model, but it is a likely target for understanding the observed enhanced vascular response to TBI. PMID: 21801588
    10. interleukin-6, endothelin ET-1, and apoptotic Bak and Bcl-XL genes have roles in small bowel transplantation, in a swine model of ischemia and reperfusion injury PMID: 21884347
    11. ET-1 elicits a different pattern of Src family kinase (SFK) activation and might trigger a different pattern of SFK activation from that caused by ATP and UTP. PMID: 21792912
    12. Compared with the untreated group, the levels of serum ET-1 after acute myocardial infarction and reperfusion were significantly decreased in the Xuefu Zhuyu-treated group. PMID: 18405606
    13. study suggests a possible role for endothelin-1(resulting from the actions of endothelin-converting enzyme-1) acting via endothelin receptor A in the control of luteolytic sensitivity in the pig PMID: 19783117
    14. vasoconstrictor influence of endogenous as well as exogenous ET on coronary circulation in vivo is reduced in myocardial infarct PMID: 15458948
    15. Data show the presence of a continuative and differentially regulated stimulating mechanism of ET-1 expression during pig endotoxaemia as well as a fundamental role of ET-1 system in the cardiovascular and respiratory derangement. PMID: 16043243
    16. These data support a linkage between the proinflammatory cytokine IL-1beta and vascular dysfunction within the intestinal circulation, mediated, at least in part, by the ET system. PMID: 16782700
    17. extracellular ET-1 regulates the abundance of ET-1 mRNA in PAECs, in an ETB receptor-dependent manner, by modulating both mRNA stability and transcription via mechanisms involving receptor endocytosis and both ERK and p38 MAPK pathways PMID: 18278064
    18. Cyclosporine-A stimulates angiotensin I/II secretion by kidney glomeruli. PMID: 18331442
    19. Left ventricular unloading with intra-aortic counter pulsation prior to reperfusion reduces myocardial release of endothelin-1 and decreases infarction size in a porcine ischemia-reperfusion model. PMID: 18814226
    20. The beneficial effect of carvedilol on myocardial no-reflow could be due to its effect on ET-1 via the activation of the K(ATP) channel. PMID: 18840580
    21. is not predictive of ventricular fibrillation during acute myocardial ischemia PMID: 18844577
    22. Porcine ET-1 rapidly activates the prosurvival phosphatidylinositol 3'-OH kinase (PI3K)/AKT signaling pathway in normal and fibrotic human lung fibroblasts. PMID: 19188658
    23. Report novel pathway involving endothelin-1 and reactive oxygen species in coronary vasospasm with endothelial dysfunction. PMID: 19623039

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  • 亞細胞定位：
    Secreted.
  • 蛋白家族：
    Endothelin/sarafotoxin family
  • 數(shù)據(jù)庫鏈接：