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Pig free thyroxine (FT4) ELISA kit

  • 中文名稱:
    豬游離甲狀腺素(FT4)酶聯(lián)免疫試劑盒
  • 貨號(hào):
    CSB-EQF027512PI
  • 規(guī)格:
    96T/48T
  • 價(jià)格:
    ¥4200/¥3000
  • 其他:

產(chǎn)品詳情

  • 產(chǎn)品描述:
    豬游離甲狀腺素(FT4)酶聯(lián)免疫試劑盒(CSB-EQF027512PI)為競爭法ELISA試劑盒,定量檢測(cè)血清、血漿、組織培養(yǎng)上清液、組織勻漿樣本中的FT4含量。FT4即游離甲狀腺素,是甲狀腺功能檢查重要指標(biāo)。它可直接反映甲狀腺功能狀態(tài)。研究機(jī)制方面,F(xiàn)T4參與機(jī)體代謝調(diào)節(jié),其水平變化與甲狀腺疾病如甲亢、甲減密切相關(guān),檢測(cè)它有助于準(zhǔn)確診斷和病情判斷。試劑盒檢測(cè)范圍為4 pmol/L-64 pmol/L,靈敏度為1.08 pmol/L??蒲腥藛T可將其應(yīng)用于動(dòng)物營養(yǎng)代謝研究、環(huán)境應(yīng)激評(píng)估、甲狀腺相關(guān)疾病模型構(gòu)建等領(lǐng)域,尤其適用于需要?jiǎng)討B(tài)監(jiān)測(cè)甲狀腺功能變化的實(shí)驗(yàn)設(shè)計(jì),如激素分泌調(diào)控、代謝性疾病機(jī)制研究或毒理學(xué)評(píng)價(jià)等,為畜牧獸醫(yī)研究及生物醫(yī)學(xué)基礎(chǔ)科研提供標(biāo)準(zhǔn)化檢測(cè)工具。本品僅用于科研,不用于臨床診斷,產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 縮寫:
    FT4
  • 種屬:
    Sus scrofa (Pig)
  • 樣本類型:
    serum, plasma, cell culture supernates, tissue homogenates
  • 檢測(cè)范圍:
    4 pmol/L-64 pmol/L
  • 靈敏度:
    1.08 pmol/L
  • 反應(yīng)時(shí)間:
    1-5h
  • 樣本體積:
    50-100ul
  • 檢測(cè)波長:
    450 nm
  • 研究領(lǐng)域:
    Others
  • 測(cè)定原理:
    quantitative
  • 測(cè)定方法:
    Competitive
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<15%        
    Three samples of known concentration were tested twenty times on one plate to assess.    
    Inter-assay Precision (Precision between assays): CV%<15%        
    Three samples of known concentration were tested in twenty assays to assess.      
                   
  • 線性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of pig FT4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.  
      Sample Serum(n=4)    
    1:1 Average % 92    
    Range % 85-99    
    1:2 Average % 95    
    Range % 87-106    
    1:4 Average % 96    
    Range % 90-110    
    1:8 Average % 91    
    Range % 86-98    
  • 回收率:
    The recovery of pig FT4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.  
     
    Sample Type Average % Recovery Range    
    Serum (n=5) 97 93-103    
    EDTA plasma (n=4) 96 93-102    
                   
                   
  • 標(biāo)準(zhǔn)曲線:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.  
     
    pmol/L OD1 OD2 Average      
    4 2.771 2.736 2.754      
    8 1.459 1.442 1.451      
    16 0.827 0.865 0.846      
    32 0.575 0.565 0.570      
    64 0.318 0.307 0.313      
  • 數(shù)據(jù)處理:
  • 貨期:
    3-5 working days

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 最新研究進(jìn)展:
    FT4指的是游離甲狀腺素,是指在血液中不與蛋白質(zhì)結(jié)合的甲狀腺素。最新研究發(fā)現(xiàn),F(xiàn)T4水平與代謝綜合征、糖尿病、心血管疾病等疾病密切相關(guān)。一項(xiàng)針對(duì)14,107名美國成年人的研究表明,F(xiàn)T4水平與冠狀動(dòng)脈鈣化風(fēng)險(xiǎn)呈正相關(guān)關(guān)系。此外,一些研究表明,孕期FT4水平異常與胎兒神經(jīng)發(fā)育相關(guān)的基因表達(dá)水平有關(guān),提示FT4水平對(duì)胎兒神經(jīng)發(fā)育的影響。