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  4. Pig glial fibrillary acidic protein,GFAP ELISA Kit

Pig glial fibrillary acidic protein,GFAP ELISA Kit

  • 中文名稱：
    豬神經膠質纖維酸性蛋白(GFAP)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-E14950p
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥4200/￥3000
  • 其他：

產品詳情

  • 產品描述：
    豬神經膠質纖維酸性蛋白(GFAP)酶聯(lián)免疫試劑盒（CSB-E14950p）為競爭法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的GFAP含量。GFAP（膠質纖維酸性蛋白）是一種特定于中樞神經系統(tǒng)的III類中間絲蛋白，主要在星形膠質細胞中表達。其研究機制涉及基因表達調控、蛋白結構、功能以及與多種神經系統(tǒng)疾病的關系，如阿爾茨海默病和亞歷山大病。GFAP在維持星形膠質細胞形態(tài)、血腦屏障形成和突觸功能中發(fā)揮重要作用。試劑盒檢測范圍為0.1 ng/mL-20 ng/mL，本試劑盒適用于豬模型在神經退行性疾病研究（如阿爾茨海默病、帕金森病）、腦創(chuàng)傷模型評估、神經毒性實驗或神經炎癥機制探索等科研場景中，定量分析血液或組織樣本的GFAP動態(tài)變化，為神經病理學研究提供可靠工具。本品僅用于科研，不用于臨床診斷，產品具體參數(shù)及操作步驟詳見產品說明書。
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Sus scrofa (Pig)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    0.1 ng/mL-20 ng/mL
  • 靈敏度：
    0.05 ng/mL
  • 反應時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領域：
    Neuroscience
  • 測定原理：
    quantitative
  • 測定方法：
    Competitive
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<15%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<15%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of pig GFAP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:1 Average % 92  
    Range % 84-97  
    1:2 Average % 96  
    Range % 92-99  
    1:4 Average % 92  
    Range % 89-96  
    1:8 Average % 96  
    Range % 90-102  
  • 回收率：
    The recovery of pig GFAP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 98 94-105  
    EDTA plasma (n=4) 96 92-103  
                 
                 
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average    
    20 0.187 0.195 0.191    
    5 0.338 0.346 0.342    
    1.6 0.559 0.551 0.555    
    0.4 0.842 0.868 0.855    
    0.1 1.334 1.337 1.336    
    0 1.989 2.005 1.997    
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產品評價

相關問答

■ 常見問題解答
Q:

I have the question about the antibody of this kit since it is being compared to another Elisa kit.
1) Is this a GFAP antibody that recognizes the full length or just the C terminus?
2) The source of the GFAP antibody ? Is it from cow spinal cord?

A:
Thanks for your inquiry.
CSB-E14950p
This kit is designed against full length protein.
The plate is coated with goat-anti-rabbit antibody. The specific antibody used in the kit is rabbit polyclonal antibody.Pls let me know if you have any further questions. Thank you.