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  4. Pig growth hormone,GH ELISA Kit

Pig growth hormone,GH ELISA Kit

  • 中文名稱：
    豬生長激素(GH)酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-E06813p
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥4200/￥3000
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    豬生長激素(GH)酶聯(lián)免疫試劑盒（CSB-E06813p）為競爭法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的GH1含量。GH1 基因編碼生長激素，生長激素在人體生長發(fā)育、代謝調(diào)節(jié)等方面至關(guān)重要。其相關(guān)研究機(jī)制圍繞生長激素的分泌調(diào)節(jié)、信號(hào)通路、功能作用等展開。如對其分泌調(diào)控機(jī)制的研究，有助于了解生長發(fā)育異常疾病，為臨床治療提供思路。試劑盒檢測范圍為2.5 ng/mL-100 ng/mL，適用于動(dòng)物生理學(xué)、營養(yǎng)代謝研究及生長調(diào)控機(jī)制探索等科研領(lǐng)域?？蔀樾竽琉B(yǎng)殖優(yōu)化、飼料效能評(píng)估等研究提供可靠數(shù)據(jù)支持。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    GH1 ELISA Kit; Somatotropin ELISA Kit; Growth hormone ELISA Kit
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Sus scrofa (Pig)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    2.5 ng/mL-100 ng/mL
  • 靈敏度：
    2.5 ng/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Signal Transduction
  • 測定原理：
    quantitative
  • 測定方法：
    Competitive
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<15%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<15%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of pig GH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:1 Average % 92  
    Range % 87-96  
    1:2 Average % 95  
    Range % 89-100  
    1:4 Average % 94  
    Range % 88-99  
    1:8 Average % 95  
    Range % 90-103  
  • 回收率：
    The recovery of pig GH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 96 90-102  
    EDTA plasma (n=4) 98 92-103  
                 
                 
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average    
    0 1.905 1.834 1.870    
    2.5 1.298 1.282 1.290    
    7.5 1.024 1.004 1.014    
    23 0.739 0.722 0.731    
    50 0.567 0.531 0.549    
    100 0.342 0.338 0.340    
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    Plays an important role in growth control. Its major role in stimulating body growth is to stimulate the liver and other tissues to secrete IGF-1. It stimulates both the differentiation and proliferation of myoblasts. It also stimulates amino acid uptake and protein synthesis in muscle and other tissues.
  • 亞細(xì)胞定位：
    Secreted.
  • 蛋白家族：
    Somatotropin/prolactin family
  • 數(shù)據(jù)庫鏈接：