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  4. Pig Insulin-like growth factor 1, IGF-1 ELISA Kit

Pig Insulin-like growth factor 1, IGF-1 ELISA Kit

  • 中文名稱：
    豬胰島素樣生長因子1(IGF-1)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-E06829p
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥3800/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    豬胰島素樣生長因子1(IGF-1)酶聯(lián)免疫試劑盒（CSB-E06829p）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織培養(yǎng)上清液、組織勻漿、細胞裂解物樣本中的IGF1含量。IGF1即胰島素樣生長因子1，在人體生長發(fā)育、細胞增殖分化等過程中起著關鍵作用。研究機制方面，它主要通過與IGF1受體結合，激活下游信號通路，影響細胞的生長、存活和代謝，在腫瘤、生長發(fā)育異常等疾病研究中受廣泛關注。試劑盒檢測范圍為6.25 ng/mL-400 ng/mL，適用于豬模型相關的基礎研究，例如生長調(diào)控機制探索、營養(yǎng)代謝實驗、肌肉發(fā)育分析或疾病模型中IGF - 1信號通路的動態(tài)監(jiān)測；為科研人員在體外實驗、動物生理研究及細胞功能分析等領域提供可靠工具，滿足實驗室對IGF - 1蛋白定量檢測的多樣化需求。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    IGF1 ELISA Kit; Insulin-like growth factor I ELISA Kit; IGF-I ELISA Kit; Somatomedin ELISA Kit
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Sus scrofa (Pig)
  • 樣本類型：
    serum, plasma, cell culture supernates, tissue homogenates, cell lysates
  • 檢測范圍：
    6.25 ng/mL-400 ng/mL
  • 靈敏度：
    1.56 ng/mL
  • 反應時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領域：
    Signal Transduction
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of pig IGF-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:1 Average % 85  
    Range % 80-98  
    1:2 Average % 95  
    Range % 90-104  
    1:4 Average % 97  
    Range % 92-110  
    1:8 Average % 95  
    Range % 82-100  
  • 回收率：
    The recovery of pig IGF-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 93 85-108  
    EDTA plasma (n=4) 95 91-105  
                 
                 
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average Corrected  
    400 2.824 2.815 2.820 2.676  
    200 2.362 2.346 2.354 2.210  
    100 1.469 1.489 1.479 1.335  
    50 0.709 0.684 0.697 0.553  
    25 0.416 0.425 0.421 0.277  
    12.5 0.251 0.262 0.257 0.113  
    6.25 0.199 0.192 0.196 0.052  
    0 0.146 0.142 0.144 　  
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

引用文獻

產(chǎn)品評價

靶點詳情

  • 功能：
    The insulin-like growth factors, isolated from plasma, are structurally and functionally related to insulin but have a much higher growth-promoting activity. May be a physiological regulator of [1-14C]-2-deoxy-D-glucose (2DG) transport and glycogen synthesis in osteoblasts. Stimulates glucose transport in bone-derived osteoblastic (PyMS) cells and is effective at much lower concentrations than insulin, not only regarding glycogen and DNA synthesis but also with regard to enhancing glucose uptake. May play a role in synapse maturation. Ca(2+)-dependent exocytosis of IGF1 is required for sensory perception of smell in the olfactory bulb. Acts as a ligand for IGF1R. Binds to the alpha subunit of IGF1R, leading to the activation of the intrinsic tyrosine kinase activity which autophosphorylates tyrosine residues in the beta subunit thus initiatiating a cascade of down-stream signaling events leading to activation of the PI3K-AKT/PKB and the Ras-MAPK pathways. Binds to integrins ITGAV:ITGB3 and ITGA6:ITGB4. Its binding to integrins and subsequent ternary complex formation with integrins and IGFR1 are essential for IGF1 signaling. Induces the phosphorylation and activation of IGFR1, MAPK3/ERK1, MAPK1/ERK2 and AKT1.
  • 基因功能參考文獻：
    1. High temperature, independent feed intake reduction, increases IGF-1:IGFBP-3 ratio. PMID: 30196890
    2. the dipeptide Pro-Asp promoted IGF-1 secretion and expression in hepatocytes. PMID: 27473671
    3. The immunoprecipitation results also showed that high AA concentrations significantly increased the interaction of mTOR and PPARg. In summary, PPARg plays an important role in the regulation of IGF-1 secretion and gene expression in response to dietary protein. PMID: 28257428
    4. Data suggest that both ovarian follicular dominance in cows and cooperation of ovarian follicles in pigs can be mediated by either down- or up-regulation of the insulin-like growth factor 1/oxytocin system. PMID: 28163019
    5. This study demonstrated that Up-regulation of IGF-1 in the frontal cortex of piglets exposed to an environmentally enriched arena. PMID: 28238777
    6. Combined administration of mesenchymal stem cells overexpressing IGF-1 and HGF enhances neovascularization but moderately improves cardiac regeneration in a porcine model. PMID: 27423905
    7. Results show that expression changes of IGF1 genes were associated with C/EBP b expression during embryonic and postnatal development in porcine liver. PMID: 25543525
    8. genetic association study suggests that the TC genotype of IGF-1 represents the selection genotype for smaller in size. PMID: 24634183
    9. IGF-I coordinately regulates multiple cell signaling pathways that are critical to proliferation, migration and survival of trophectoderm cells during early pregnancy in pigs. PMID: 24508636
    10. IGF-1 splice variant (mechano growth factor) is expressed within the growth plate, but the addition of its peptides was not associated with growth plate chondrocytes proliferation. PMID: 24146828
    11. alpha-linolenic acid increased IGF-I secretion from hepatocytes. PMID: 23053988
    12. Copper can stimulate chondrocyte proliferation by promoting the expression of IGF-1. PMID: 22367704
    13. The results show that modulation of plasma insulin levels by dietary carbohydrates seems possible in anabolic sows, but IGF-I levels are less easily modified. PMID: 21635573
    14. The results suggested that IFG-1 and -2 and their receptors are differentially expressed at the maternal and fetal components of the attachment site. PMID: 21685713
    15. Plasma ghrelin level and myocardial IGF-1 mRNA expression were significantly up-regulated, while plasma IGF-1 level and myocardial ghrelin mRNA expression were down-regulated in the chronic cyanotic immature piglets. PMID: 21933568
    16. IGFI signaling pathway regulates myofibre hypertrophy postnatally via complicated signal effectors, which may have negative impact on meat quality simultaneously. PMID: 21246287
    17. IGF-I, IGF-II, and IGFBP-3 mRNA were positively correlated with IGF-IR from 50E to 180D, suggesting that the expression of IGF-system genes exhibits specific developmental patterns in the skeletal muscle tissues. PMID: 21570979
    18. These results indicate that sorbic acid improves growth performance by regulating IGF system gene expression and hormone secretion. PMID: 21421836
    19. In an animal model of acute myocardial infarction relevant to human disease, intracoronary administration of IGF-1/hepatocyte growth factor (HGF) is a practical and effective strategy to reduce pathological cardiac remodeling. PMID: 21723061
    20. The expression of IGF-I and IGF-II in native growth plates of prepubertal piglets and under different cell culture conditions, was compared. PMID: 20600587
    21. The aims of this study were to investigate whether aldosterone influences IGF-I signaling and to determine the mechanism(s) by which aldosterone affects IGF-I function. PMID: 20881255
    22. Data show that hepatocytes secreted IGF-I under basal conditions and this was enhanced by GH addition. PMID: 19786114
    23. an important role of IGF1 in leptin receptor expression during the entire estrous cycle, with a special role during the prepubertal period PMID: 17139134
    24. study concludes that IGF1 promotes the FSH-stimulated synthesis and retention of hyaluronic acid in pig oocyte-cumulus complexes by PIK3/AKT- and MAPK3/1-dependent mechanisms PMID: 17329596
    25. Significant effect was found for IGF1 genotype on birth weight (P<0.05), with positive effects associated with the 286/286 genotype, and 280/286 genotype on litter weight at birth in second parity. PMID: 17369178
    26. Both classes of IGF-I mRNA were expressed in a variety of tissues, however, class 1 IGF-I mRNA was more abundant than class 2 in all tissues. PMID: 18157703
    27. in VSMCs exposed to hyperglycemia, IGF-I stimulation of Shc facilitates the transfer of Grb2 to p85 resulting in enhanced PI3K activation and AKT phosphorylation leading to enhanced cell proliferation and migration PMID: 18420583
    28. These data demonstrated that feed allowance level has important consequence on embryo survival, embryo development and uterine development, possibly mediated by changes in concentrations of reproductive hormones. PMID: 18700853

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  • 亞細胞定位：
    Secreted.
  • 蛋白家族：
    Insulin family
  • 數(shù)據(jù)庫鏈接：