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  4. Pig insulin-like growth factors 2,IGF-2 ELISA Kit

Pig insulin-like growth factors 2,IGF-2 ELISA Kit

  • 中文名稱：
    豬胰島素樣生長因子2(IGF-2)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-E06830p
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥3800/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    豬胰島素樣生長因子2(IGF-2)酶聯(lián)免疫試劑盒（CSB-E06830p）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿樣本中的IGF2含量。IGF2即胰島素樣生長因子 2，是一種具有重要生物學(xué)功能的蛋白質(zhì)。它參與胎兒生長發(fā)育、細(xì)胞增殖分化等過程。研究中，IGF2在腫瘤發(fā)生發(fā)展機(jī)制里受關(guān)注，與腫瘤細(xì)胞的生長、存活、侵襲等密切相關(guān)，是潛在的腫瘤治療靶點(diǎn)。試劑盒檢測范圍為0.625 ng/mL-40 ng/mL，適用于畜牧科學(xué)研究中動物生長性能評估、飼料效能分析、疾病狀態(tài)下的代謝變化研究等場景，特別針對豬只生長發(fā)育監(jiān)測、繁殖生理研究及應(yīng)激反應(yīng)機(jī)制探討提供可靠檢測方案本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    IGF2Insulin-like growth factor II ELISA Kit; IGF-II) [Cleaved into: Insulin-like growth factor II; Preptin] ELISA Kit
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Sus scrofa (Pig)
  • 樣本類型：
    serum, plasma
  • 檢測范圍：
    0.625 ng/mL-40 ng/mL
  • 靈敏度：
    0.156 ng/mL
  • 反應(yīng)時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Metabolism
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of pig IGF-2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)
    1:20 Average % 89
    Range % 83-97
    1:40 Average % 94
    Range % 90-103
    1:80 Average % 92
    Range % 86-100
    1:160 Average % 93
    Range % 87-101
  • 回收率：
    The recovery of pig IGF-2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 97 91-105
    EDTA plasma (n=4) 95 89-105
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average Corrected
    40 2.754 2.714 2.734 2.565
    20 2.144 2.126 2.135 1.966
    10 1.439 1.457 1.448 1.279
    5 0.925 0.932 0.929 0.760
    2.5 0.525 0.533 0.529 0.360
    1.25 0.381 0.364 0.373 0.204
    0.625 0.255 0.249 0.252 0.083
    0 0.170 0.168 0.169 　
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評價(jià)

靶點(diǎn)詳情

  • 功能：
    The insulin-like growth factors possess growth-promoting activity. Major fetal growth hormone in mammals. Plays a key role in regulating fetoplacental development. IGF2 is influenced by placental lactogen. Also involved in tissue differentiation. In adults, involved in glucose metabolism in adipose tissue, skeletal muscle and liver. Acts as a ligand for integrin which is required for IGF2 signaling. Positively regulates myogenic transcription factor MYOD1 function by facilitating the recruitment of transcriptional coactivators, thereby controlling muscle terminal differentiation. Inhibits myoblast differentiation and modulates metabolism via increasing the mitochondrial respiration rate.; Preptin undergoes glucose-mediated co-secretion with insulin, and acts as physiological amplifier of glucose-mediated insulin secretion. Exhibits osteogenic properties by increasing osteoblast mitogenic activity through phosphoactivation of MAPK1 and MAPK3.
  • 基因功能參考文獻(xiàn)：
    1. These results indicated that editing IGF2 intron 3-3072 site using CRISPR-Cas9 technology improved meat production in Bama pigs. PMID: 30259067
    2. trans-associations occur between three imprinted genes IGF2, DLK1 and MEG3 both in fetal liver and muscle cells. PMID: 27716032
    3. A single nucleotide polymorphism in a regulatory region of intron 3 within the porcine IGF2 gene is associated with increased lean deposition and decreased fat deposition in pigs with paternal A alleles compared with pigs with paternal G alleles. PMID: 25367517
    4. These findings indicated that the mRNA of H19 and IGF2 genes is susceptible to in vitro environments during the process of ES cell derivation from blastocysts but DNA methylation status at this region was well maintained. PMID: 24794444
    5. study did not find any significant associations for polymorphisms in insulin-like grwoth factor 2, GTP Binding Protein alpha Subunits, Gs and melanocortin receptor 4 genes with reproductive traits of Polish Landrace and Large White pigs PMID: 23199214
    6. The results suggested that IFG-1 and -2 and their receptors are differentially expressed at the maternal and fetal components of the attachment site. PMID: 21685713
    7. Genetic variation in the promoter region of the IGF2 gene is associated with intramuscular fat content in porcine skeletal muscle. PMID: 21779802
    8. The IGF2 G allele has strong adipogenic effects at the subcutaneous adipose tissue level and enhances the intermuscular fat content in ham. PMID: 21907500
    9. IGF-I, IGF-II, and IGFBP-3 mRNA were positively correlated with IGF-IR from 50E to 180D, suggesting that the expression of IGF-system genes exhibits specific developmental patterns in the skeletal muscle tissues. PMID: 21570979
    10. The imprinting status in adult liver, muscle and kidney tissues were also not reflected in the methylation patterns of IGF2 DMRs 1 and 2. PMID: 21575277
    11. The expression of IGF-I and IGF-II in native growth plates of prepubertal piglets and under different cell culture conditions, was compared. PMID: 20600587
    12. The aim of this work was to study the effects on litter size of variants of the porcine genes RBP4, ESR1 and IGF2, currently used in genetic tests for different purposes. PMID: 20576168
    13. Promoter-specific expression differences of IGF2 isoforms and IGF2AS between biparental and parthenogenetic swine fetal tissues were documented. IGF2 and IGF2AS are paternally expressed. PMID: 19571260
    14. In both skeletal muscle and heart muscle growth, the insulin-like growth factor-2:myostatin interaction seems to play an important role. PMID: 19674865
    15. Effect of genotype on proteolytic enzyme in skeletal muscles during growth. PMID: 15560245
    16. Mutation controls the paternally expressed QTL for backfat thickness in a cross between Meishan and European Whites. PMID: 15678747
    17. A significant effect of linkage disequilibrium between IGF2-in3-G3072A and IGF2-in7-G162C on backfat thickness and lean meat content was found. PMID: 16706926
    18. Results showed that in IGF2 gene, the predominant allele was C, and the predominant genotype was CC. and that Wuzhishan pig breed was at Hardy-Weinberg equilibrium with respect to this SNP. PMID: 17284425
    19. The majority of IGF2 transcripts are expressed from promoters 2-4 and are imprinted. PMID: 18228173
    20. IGF2 gene has significant effects on individual birth weight and backfat thickness PMID: 18244923
    21. Haplotype variability around a known causative mutation in porcine gene IGF2, which increases lean content, is analysed. PMID: 18245828
    22. The results suggest that the expression of IGF2 and IGFBP3 mRNA in the adipose tissue of pigs exhibits specific developmental changes and different patterns between the two pig breeds. PMID: 18487150
    23. The current findings suggest that IGF-I and IGF-II have different effects on porcine adipose cell development. PMID: 18640238
    24. Methylation status of differentially methylated regions of Igf2/H19 locus in porcine gametes and preimplantation embryos are reported. PMID: 18983907
    25. The selection based on the IGF2 mutation in Poland may be very useful in Polish Landrace and Large White pigs, where the G allele is still relatively frequent. PMID: 19638682

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  • 亞細(xì)胞定位：
    Secreted.
  • 蛋白家族：
    Insulin family
  • 數(shù)據(jù)庫鏈接：

    KEGG: ssc:396916

    UniGene: Ssc.9365