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Pig interleukin 17,IL-17 ELISA Kit

  • 中文名稱:
    豬白介素17(IL-17)酶聯(lián)免疫試劑盒
  • 貨號(hào):
    CSB-E08057p
  • 規(guī)格:
    96T/48T
  • 價(jià)格:
    ¥3800/¥2500
  • 其他:

產(chǎn)品詳情

  • 產(chǎn)品描述:
    豬白介素17(IL-17)酶聯(lián)免疫試劑盒(CSB-E08057p)為雙抗夾心法ELISA試劑盒,定量檢測(cè)血清、血漿、組織勻漿樣本中的IL17A含量。IL17A是一種重要的細(xì)胞因子。其背景在于它在多種炎癥性疾病及自身免疫病病理過(guò)程中扮演關(guān)鍵角色。研究機(jī)制上,IL17A能調(diào)節(jié)免疫細(xì)胞功能、促進(jìn)炎性介質(zhì)分泌等,以此影響疾病發(fā)生發(fā)展,是當(dāng)前免疫和炎癥領(lǐng)域研究熱點(diǎn)。試劑盒檢測(cè)范圍為20 pg/mL- 800 pg/mL,適用于體外實(shí)驗(yàn)環(huán)境,可穩(wěn)定檢測(cè)多種生物樣本中IL - 17的表達(dá)水平,為動(dòng)物免疫學(xué)、病原微生物感染研究以及抗炎藥物開(kāi)發(fā)提供可靠數(shù)據(jù)支持。IL - 17的動(dòng)態(tài)監(jiān)測(cè)對(duì)豬類免疫機(jī)制研究、炎癥性疾病模型構(gòu)建及疫苗效果評(píng)估具有重要科研價(jià)值。本品僅用于科研,不用于臨床診斷,產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書(shū)。
  • 別名:
    IL17A ELISA Kit; IL17 ELISA Kit; Interleukin-17A ELISA Kit; IL-17 ELISA Kit; IL-17A ELISA Kit
  • 縮寫(xiě):
  • Uniprot No.:
  • 種屬:
    Sus scrofa (Pig)
  • 樣本類型:
    serum, plasma, tissue homogenates
  • 檢測(cè)范圍:
    20 pg/mL- 800 pg/mL
  • 靈敏度:
    10 pg/mL
  • 反應(yīng)時(shí)間:
    1-5h
  • 樣本體積:
    50-100ul
  • 檢測(cè)波長(zhǎng):
    450 nm
  • 研究領(lǐng)域:
    Immunology
  • 測(cè)定原理:
    quantitative
  • 測(cè)定方法:
    Sandwich
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<15%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<15%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of pig IL-17 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)  
    1:1 Average % 90  
    Range % 86-96  
    1:2 Average % 99  
    Range % 92-106  
    1:4 Average % 89  
    Range % 83-94  
    1:8 Average % 92  
    Range % 87-98  
  • 回收率:
    The recovery of pig IL-17 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 94 90-99  
    EDTA plasma (n=4) 84 80-88  
                 
                 
  • 標(biāo)準(zhǔn)曲線:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average    
    800 2.806 2.862 2.834    
    400 2.080 2.097 2.089    
    200 1.174 1.198 1.186    
    100 0.698 0.663 0.681    
    40 0.281 0.294 0.288    
    20 0.147 0.145 0.146    
  • 數(shù)據(jù)處理:
  • 貨期:
    3-5 working days

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能:
    Effector cytokine of innate and adaptive immune system involved in antimicrobial host defense and maintenance of tissue integrity. Signals via IL17RA-IL17RC heterodimeric receptor complex, triggering homotypic interaction of IL17RA and IL17RC chains with TRAF3IP2 adapter. This leads to downstream TRAF6-mediated activation of NF-kappa-B and MAPkinase pathways ultimately resulting in transcriptional activation of cytokines, chemokines, antimicrobial peptides and matrix metalloproteinases, with potential strong immune inflammation. Plays an important role in connecting T cell-mediated adaptive immunity and acute inflammatory response to destroy extracellular bacteria and fungi. As a signature effector cytokine of T-helper 17 cells (Th17), primarily induces neutrophil activation and recruitment at infection and inflammatory sites. In airway epithelium, mediates neutrophil chemotaxis via induction of CXCL1 and CXCL5 chemokines. In secondary lymphoid organs, contributes to germinal center formation by regulating the chemotactic response of B cells to CXCL12 and CXCL13, enhancing retention of B cells within the germinal centers, B cell somatic hypermutation rate and selection toward plasma cells. Effector cytokine of a subset of gamma-delta T cells that functions as part of an inflammatory circuit downstream IL1B, TLR2 and IL23A-IL12B to promote neutrophil recruitment for efficient bacterial clearance. Effector cytokine of innate immune cells including invariant natural killer cell (iNKT) and group 3 innate lymphoid cells that mediate initial neutrophilic inflammation. Involved in the maintenance of the integrity of epithelial barriers during homeostasis and pathogen infection. Upon acute injury, has a direct role in epithelial barrier formation by regulating OCLN localization and tight junction biogenesis. As part of the mucosal immune response induced by commensal bacteria, enhances host's ability to resist pathogenic bacterial and fungal infections by promoting neutrophil recruitment and antimicrobial peptides release. In synergy with IL17F, mediates the production of antimicrobial beta-defensins DEFB1, DEFB103A, and DEFB104A by mucosal epithelial cells, limiting the entry of microbes through the epithelial barriers. Involved in antiviral host defense through various mechanisms. Enhances immunity against West Nile virus by promoting T cell cytotoxicity. May play a beneficial role in influenza A virus (H5N1) infection by enhancing B cell recruitment and immune response in the lung. Contributes to influenza A virus (H1N1) clearance by driving the differentiation of B-1a B cells, providing for production of virus-specific IgM antibodies at first line of host defense.
  • 基因功能參考文獻(xiàn):
    1. At week 15, skin cell infiltrates from pigs with crusted scabies had significantly higher CD8+ T cell, gammadelta T cell and IL-17+ cell numbers than those with ordinary scabies. Peripheral IL-17 levels were not increased, suggesting that localized skin IL-17-secreting T cells may play a critical role in the pathogenesis of crusted scabies development. PMID: 25040151
    2. Alterations in the IL-17 regulated pathway of porcine colon in response to dietary supplementation with seaweeds and yeast are reported. PMID: 23365350
    3. conclude that only two subpopulations of porcine WBCs are sources of IL-17 after non-specific stimulation: CD3(+)CD4(+) and CD3(+)gammadeltaTCR(+). PMID: 22321808
    4. Cloning and expression of interleukin-17. PMID: 15450131
  • 亞細(xì)胞定位:
    Secreted.
  • 蛋白家族:
    IL-17 family
  • 組織特異性:
    Highly expressed in adult heart, skin, and intestinal tissues, such as jejunum and ileum.
  • 數(shù)據(jù)庫(kù)鏈接: