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  4. Pig interleukin 2,IL-2 ELISA Kit

Pig interleukin 2,IL-2 ELISA Kit

  • 中文名稱：
    豬白介素2(IL-2)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-E06783p
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    豬白介素2(IL-2)酶聯(lián)免疫試劑盒（CSB-E06783p）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的IL2含量。IL2是重要的免疫細胞因子，在免疫調(diào)節(jié)中發(fā)揮關(guān)鍵作用。其背景在于它能促進T細胞、NK細胞等免疫細胞增殖與活化。研究機制上，IL2通過與受體結(jié)合激活下游信號通路，增強機體免疫功能，在腫瘤免疫治療等領(lǐng)域研究廣泛。試劑盒檢測范圍為15.6 pg/mL-1000 pg/mL，適用于科研領(lǐng)域中對豬類免疫系統(tǒng)功能的評估，例如疫苗研發(fā)中免疫激活效果分析、傳染病模型中細胞免疫反應(yīng)監(jiān)測，或通過檢測組織內(nèi)IL - 2表達水平探究炎癥性疾病的分子機制。為畜牧獸醫(yī)研究、比較免疫學(xué)及動物疾病模型構(gòu)建提供可靠工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    IL2Interleukin-2 ELISA Kit; IL-2 ELISA Kit; T-cell growth factor ELISA Kit; TCGF ELISA Kit
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Sus scrofa (Pig)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    15.6 pg/mL-1000 pg/mL
  • 靈敏度：
    3.9 pg/mL
  • 反應(yīng)時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Immunology
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of pig IL-2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %92
    Range %80-99
    1:2Average %86
    Range %82-91
    1:4Average %91
    Range %86-97
    1:8Average %94
    Range %88-98
  • 回收率：
    The recovery of pig IL-2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9489-102
    EDTA plasma (n=4)9691-102
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    10002.884 2.915 2.900 2.764
    5002.123 2.104 2.114 1.978
    2501.249 1.272 1.261 1.125
    1250.818 0.846 0.832 0.696
    62.50.492 0.440 0.466 0.330
    31.20.314 0.322 0.318 0.182
    15.60.263 0.258 0.261 0.125
    00.135 0.136 0.136 　
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評價

靶點詳情

  • 功能：
    Produced by T-cells in response to antigenic or mitogenic stimulation, this protein is required for T-cell proliferation and other activities crucial to regulation of the immune response. Can stimulate B-cells, monocytes, lymphokine-activated killer cells, natural killer cells, and glioma cells.
  • 基因功能參考文獻：
    1. We observed that besides TCR stimulation Tregs require IL-2 for activation. PMID: 21530576
    2. a parameter of early mRNA expression during intestinal ischemia reperfusion injury PMID: 15050130
    3. The isolation and characterization of interleukin 2 from Tibetan swine are reported. PMID: 16195934
  • 亞細胞定位：
    Secreted.
  • 蛋白家族：
    IL-2 family
  • 數(shù)據(jù)庫鏈接：