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  4. Pig interleukin 4,IL-4 ELISA Kit

Pig interleukin 4,IL-4 ELISA Kit

  • 中文名稱：
    豬白介素4(IL-4)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-E06785p
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    豬白介素4(IL-4)酶聯(lián)免疫試劑盒（CSB-E06785p）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織培養(yǎng)上清液、組織勻漿樣本中的IL4含量。IL4是重要的靶點(diǎn)。它是白細(xì)胞介素家族成員，參與免疫調(diào)節(jié)、炎癥反應(yīng)等多種生理病理過程。研究機(jī)制上，IL4與其受體結(jié)合后，激活細(xì)胞內(nèi)信號通路，影響Th2細(xì)胞分化等。對其研究有助于理解相關(guān)疾病發(fā)病機(jī)制及開發(fā)治療藥物。試劑盒檢測范圍為6.25 pg/mL-400 pg/mL，該產(chǎn)品可廣泛應(yīng)用于豬免疫相關(guān)疾病模型構(gòu)建、疫苗效力評估、炎癥機(jī)制研究等科研領(lǐng)域，為探索IL - 4在感染性疾病、自身免疫性疾病及過敏性反應(yīng)中的作用提供可靠工具，尤其適用于比較醫(yī)學(xué)研究和畜牧獸醫(yī)領(lǐng)域的免疫學(xué)實(shí)驗(yàn)需求。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    IL4Interleukin-4 ELISA Kit; IL-4 ELISA Kit; B-cell stimulatory factor 1 ELISA Kit; BSF-1 ELISA Kit; Lymphocyte stimulatory factor 1 ELISA Kit
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Sus scrofa (Pig)
  • 樣本類型：
    serum, plasma, cell culture supernates, tissue homogenates
  • 檢測范圍：
    6.25 pg/mL-400 pg/mL
  • 靈敏度：
    1.56 pg/mL
  • 反應(yīng)時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Immunology
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of pig IL-4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %86
    Range %82-91
    1:2Average %90
    Range %85-95
    1:4Average %89
    Range %84-93
    1:8Average %96
    Range %92-101
  • 回收率：
    The recovery of pig IL-4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 10095-105
    EDTA plasma (n=4)9286-96
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    4002.719 2.652 2.686 2.492
    2001.962 1.861 1.912 1.718
    1001.213 1.204 1.209 1.015
    500.748 0.689 0.719 0.525
    250.551 0.527 0.539 0.345
    12.50.355 0.374 0.365 0.171
    6.250.295 0.272 0.284 0.090
    00.192 0.196 0.194 　
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評價

靶點(diǎn)詳情

  • 功能：
    Participates in at least several B-cell activation processes as well as of other cell types. It is a costimulator of DNA-synthesis. It induces the expression of class II MHC molecules on resting B-cells. It enhances both secretion and cell surface expression of IgE and IgG1. It also regulates the expression of the low affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes. Positively regulates IL31RA expression in macrophages. Stimulates autophagy in dendritic cells by interfering with mTORC1 signaling and through the induction of RUFY4.
  • 基因功能參考文獻(xiàn)：
    1. IL-4- and IL-10-producing invariant NKT cells inhibit the Th1 cell response, but not the Th17 cell response PMID: 21572032
    2. IL-4 induced activation of Akt/SREBP-1/lipid biosynthesis in EC, resulting in protection against membrane attack complex and melittin, in association with mitochondrial protection. PMID: 20017192
    3. There was no significant difference of IL-4 production between fresh and frozen peripheral blood mononuclear cells. PMID: 19766617
    4. The endothelium and subendothelium of porcine iliac arteries that are transduced with recombinant pig IL-4 are effectively protected from complement-dependent immediate injury after perfusion with human blood. PMID: 17082655
    5. unlike in humans and mice, porcine IL-4 blocks antibody and IL-6 secretion and suppresses antigen-stimulated proliferation of B cells PMID: 18761033

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  • 亞細(xì)胞定位：
    Secreted.
  • 蛋白家族：
    IL-4/IL-13 family
  • 數(shù)據(jù)庫鏈接：