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  4. Pig intestinal fatty acid binding protein,iFABP ELISA Kit

Pig intestinal fatty acid binding protein,iFABP ELISA Kit

  • 中文名稱：
    豬腸脂肪酸結(jié)合蛋白(iFABP)酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-E13994p
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥4200/￥3000
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    豬腸脂肪酸結(jié)合蛋白(iFABP)酶聯(lián)免疫試劑盒（CSB-E13994p）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿樣本中的FABP2含量。FABP2是一個(gè)靶點(diǎn)。它在脂肪酸攝取、轉(zhuǎn)運(yùn)等過程中發(fā)揮重要作用。研究聚焦于其與代謝性疾病關(guān)聯(lián)機(jī)制，如肥胖、糖尿病等。解析其結(jié)構(gòu)和功能，有助于深入了解疾病發(fā)病機(jī)制，為開發(fā)針對(duì)性治療藥物提供理論支持。試劑盒檢測范圍為78 pg/mL-5000 pg/mL，適用于腸道炎癥、缺血再灌注損傷等病理機(jī)制的科研分析；適用于科研場景中動(dòng)物模型腸道損傷的動(dòng)態(tài)監(jiān)測、營養(yǎng)干預(yù)對(duì)腸道功能影響評(píng)估，或藥物研發(fā)中腸道保護(hù)效果的量化分析；為消化道相關(guān)疾病的基礎(chǔ)研究提供標(biāo)準(zhǔn)化檢測方案。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    FABP2 ELISA Kit; FABPIFatty acid-binding protein ELISA Kit; intestinal ELISA Kit; Fatty acid-binding protein 2 ELISA Kit; Intestinal-type fatty acid-binding protein ELISA Kit; I-FABP ELISA Kit
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Sus scrofa (Pig)
  • 樣本類型：
    serum, plasma
  • 檢測范圍：
    78 pg/mL-5000 pg/mL
  • 靈敏度：
    19.5 pg/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Signal Transduction
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of pig iFABP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:1Average %92
    Range %87-93
    1:2Average %100
    Range %97-103
    1:4Average %85
    Range %81-89
    1:8Average %93
    Range %90-96
  • 回收率：
    The recovery of pig iFABP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 8481-89
    EDTA plasma (n=4)9993-103
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    50002.129 2.138 2.134 1.976
    25001.628 1.656 1.642 1.484
    12501.084 1.087 1.086 0.928
    6250.607 0.638 0.623 0.465
    3120.445 0.426 0.436 0.278
    1560.348 0.323 0.336 0.178
    780.268 0.251 0.260 0.102
    00.157 0.159 0.158
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    FABP are thought to play a role in the intracellular transport of long-chain fatty acids and their acyl-CoA esters. FABP2 is probably involved in triglyceride-rich lipoprotein synthesis. Binds saturated long-chain fatty acids with a high affinity, but binds with a lower affinity to unsaturated long-chain fatty acids. FABP2 may also help maintain energy homeostasis by functioning as a lipid sensor.
  • 基因功能參考文獻(xiàn)：
    1. The full-length cDNA of I-FABP was cloned from intestine and the mRNA was extensively present in various tissues, but I-FABP transcript of approximately 620 bp was more abundant in intestine than in other tissues. PMID: 16529296
    2. Plasma I-FABP concentration showed great variation within treatments, and no difference was observed in plasma I-FABP concentrations between the weaned conventionally and unweaned treatments PMID: 18708596
    3. Association analyses of the FABP2:g.412T>C with fatty acid composition traits were not significant in simple association. PMID: 19207930
  • 亞細(xì)胞定位：
    Cytoplasm.
  • 蛋白家族：
    Calycin superfamily, Fatty-acid binding protein (FABP) family
  • 數(shù)據(jù)庫鏈接：