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  4. Pig major acute phase protein (MAP) ELISA Kit

Pig major acute phase protein (MAP) ELISA Kit

  • 中文名稱：
    豬主要急性期蛋白( MAP)酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-E13425p
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥4200/￥3000
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    豬主要急性期蛋白( MAP)酶聯(lián)免疫試劑盒（CSB-E13425p）為競(jìng)爭(zhēng)法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的ITIH4含量。試劑盒檢測(cè)范圍為37.5 ng/mL-2400 ng/mL，該產(chǎn)品適用于動(dòng)物免疫學(xué)研究、疾病模型建立后的病理分析，以及抗炎藥物或疫苗效果評(píng)估等科研場(chǎng)景，尤其可為豬的急性炎癥反應(yīng)機(jī)制研究、組織損傷修復(fù)實(shí)驗(yàn)提供定量檢測(cè)支持。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    ITIH4 ELISA Kit; IHRP ELISA Kit; Inter-alpha-trypsin inhibitor heavy chain H4 ELISA Kit; ITI heavy chain H4 ELISA Kit; ITI-HC4 ELISA Kit; Inter-alpha-inhibitor heavy chain 4 ELISA Kit; Inter-alpha-trypsin inhibitor family heavy chain-related protein ELISA Kit; IHRP ELISA Kit; Major acute phase protein ELISA Kit; MAP ELISA Kit
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Sus scrofa (Pig)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測(cè)范圍：
    37.5 ng/mL-2400 ng/mL
  • 靈敏度：
    9.38 ng/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測(cè)波長(zhǎng)：
    450 nm
  • 研究領(lǐng)域：
    Immunology
  • 測(cè)定原理：
    quantitative
  • 測(cè)定方法：
    Competitive
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of pig MAP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:200 Average % 97  
    Range % 92-104  
    1:400 Average % 95  
    Range % 91-99  
    1:800 Average % 93  
    Range % 87-99  
    1:1600 Average % 86  
    Range % 82-92  
  • 回收率：
    The recovery of pig MAP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 95 89-101  
    EDTA plasma (n=4) 97 90-102  
                 
                 
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average    
    2400 0.138 0.141 0.140    
    1200 0.457 0.460 0.459    
    600 0.903 0.898 0.901    
    300 1.134 1.121 1.128    
    150 1.518 1.483 1.501    
    75 1.689 1.706 1.698    
    37.5 2.032 2.049 2.041    
    0 2.187 2.114 2.151    
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    Type II acute-phase protein (APP) involved in inflammatory responses to trauma. May also play a role in liver development or regeneration.
  • 基因功能參考文獻(xiàn)：
    1. Promoter of ITIH4 major acute phase protein has been isolated, cloned, and proven functional by response to an inflammatory stimulus (interleukin-6) in human Hep3B cells. (inter-alpha-trypsin heavy chain 4; ITIH4) PMID: 16275952
  • 亞細(xì)胞定位：
    Secreted.
  • 蛋白家族：
    ITIH family
  • 組織特異性：
    Liver specific.
  • 數(shù)據(jù)庫鏈接：