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  4. Pig prolactin (PRL) ELISA Kit

Pig prolactin (PRL) ELISA Kit

  • 中文名稱：
    豬催乳素(PRL)酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-E15801p
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    豬催乳素(PRL)酶聯(lián)免疫試劑盒（CSB-E15801p）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿樣本中的PRL含量。PRL即泌乳素，是腺垂體分泌的多肽激素。其分泌受下丘腦調(diào)節(jié)，參與乳腺發(fā)育、泌乳啟動(dòng)與維持等生理過程。近年研究發(fā)現(xiàn)它還與腫瘤生長(zhǎng)、轉(zhuǎn)移相關(guān)，在腫瘤微環(huán)境中影響免疫細(xì)胞功能，成為潛在抗癌治療靶點(diǎn)，研究其機(jī)制有重要意義。試劑盒檢測(cè)范圍為0.78 ng/ml-50 ng/ml.，本試劑盒通過高特異性抗體對(duì)目標(biāo)蛋白進(jìn)行捕獲與檢測(cè)，適用于實(shí)驗(yàn)室環(huán)境下針對(duì)豬血清或血漿樣本的PRL水平分析，可服務(wù)于動(dòng)物生殖生理機(jī)制研究、泌乳相關(guān)調(diào)控通路探索以及農(nóng)業(yè)養(yǎng)殖中繁殖效率評(píng)估等科研方向。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    PRL ELISA Kit; Prolactin ELISA Kit; PRL ELISA Kit
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Sus scrofa (Pig)
  • 樣本類型：
    serum, plasma
  • 檢測(cè)范圍：
    0.78 ng/ml-50 ng/ml.
  • 靈敏度：
    0.195 ng/ml
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測(cè)波長(zhǎng)：
    450 nm
  • 研究領(lǐng)域：
    Signal Transduction
  • 測(cè)定原理：
    quantitative
  • 測(cè)定方法：
    Sandwich
  • 精密度：

    Intra-assay Precision (Precision within an assay): CV%<8%

    Three samples of known concentration were tested twenty times on one plate to assess.

    Inter-assay Precision (Precision between assays): CV%<10%

    Three samples of known concentration were tested in twenty assays to assess.

  • 線性度：

    To assess the linearity of the assay, samples were spiked with high concentrations of pig PRL in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

    　

    Sample

    Serum(n=4)

    1:1

    Average %

    99

    Range %

    87-95

    1:2

    Average %

    94

    Range %

    89-97

    1:4

    Average %

    99

    Range %

    94-102

    1:8

    Average %

    95

    Range %

    91-99

  • 回收率：

    The recovery of pig PRL spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range

    Serum (n=5)

    94

    91-97

    EDTA plasma (n=4)

    95

    92-97

  • 標(biāo)準(zhǔn)曲線：

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

    pg/ml

    OD1

    OD2

    Average

    Corrected

    50

    2.677

    2.713

    2.695

    2.495

    25

    1.886

    1.901

    1.894

    1.694

    12.5

    1.365

    1.412

    1.389

    1.189

    6.25

    1.000

    1.032

    1.016

    0.816

    3.12

    0.785

    0.792

    0.789

    0.589

    1.56

    0.546

    0.554

    0.550

    0.350

    0.78

    0.346

    0.324

    0.335

    0.135

    0

    0.199

    0.200

    0.200

    　

  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    Prolactin acts primarily on the mammary gland by promoting lactation.
  • 基因功能參考文獻(xiàn)：
    1. Prolactin and, to a lesser extent progesterone, which increase in early pregnancy, enhance basal and glucose-stimulated insulin secretion in part by increasing glucokinase activity and amplifying cAMP levels. PMID: 14559722
    2. iv infusion of prolactin primarily caused coronary, mesenteric, renal, and iliac vasoconstriction PMID: 17463060
    3. despite a lack of clear relationship between PRL/C499T polymorphism in 5' UTR of the PRL gene and plasma prolactin cioncentration, gene polymorphism impact on reproductiove performance cannot be excluded PMID: 19675522
  • 亞細(xì)胞定位：
    Secreted.
  • 蛋白家族：
    Somatotropin/prolactin family
  • 數(shù)據(jù)庫(kù)鏈接：