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  4. Pig soluble P-selectin,sP-Selectin ELISA Kit

Pig soluble P-selectin,sP-Selectin ELISA Kit

  • 中文名稱：
    豬可溶性P選擇素(sP-Selectin)酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-E08106p
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥4200/￥3000
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    豬可溶性P選擇素(sP-Selectin)酶聯(lián)免疫試劑盒（CSB-E08106p）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織培養(yǎng)上清液、組織勻漿樣本中的sP-Selectin含量。sP - Selectin 即可溶性 P - 選擇素，它在炎癥反應(yīng)、血栓形成等病理生理過程中扮演重要角色。研究機(jī)制上，它可介導(dǎo)白細(xì)胞與內(nèi)皮細(xì)胞、血小板的黏附作用，其水平變化能反映機(jī)體炎癥和血管內(nèi)皮損傷程度，在心血管疾病等研究中備受關(guān)注。試劑盒檢測范圍為12.5 ng/mL-800 ng/mL，適用于科研領(lǐng)域中對(duì)豬心血管疾病模型、炎癥機(jī)制或免疫調(diào)控相關(guān)研究，可通過血清/血漿樣本評(píng)估全身性病理狀態(tài)，結(jié)合細(xì)胞培養(yǎng)上清分析體外刺激下的分子釋放規(guī)律，或通過組織勻漿探究局部微環(huán)境中sP-Selectin的表達(dá)水平，為動(dòng)物疾病模型構(gòu)建、藥物作用機(jī)制探索及生物標(biāo)志物篩選提供可靠工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 縮寫：
    sP-Selectin
  • 種屬：
    Sus scrofa (Pig)
  • 樣本類型：
    serum, plasma, cell culture supernates, tissue homogenates
  • 檢測范圍：
    12.5 ng/mL-800 ng/mL
  • 靈敏度：
    3.12 ng/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Others
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of pig sP-Selectin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:1Average %89
    Range %85-94
    1:2Average %95
    Range %92-98
    1:4Average %87
    Range %82-90
    1:8Average %93
    Range %90-96
  • 回收率：
    The recovery of pig sP-Selectin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 8783-90
    EDTA plasma (n=4)8582-89
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2AverageCorrected
    8001.828 1.968 1.898 1.750
    4001.179 1.236 1.208 1.060
    2000.734 0.757 0.746 0.598
    1000.485 0.476 0.481 0.333
    500.311 0.323 0.317 0.169
    250.251 0.257 0.254 0.106
    12.50.188 0.182 0.185 0.037
    00.145 0.151 0.148
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

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