Pig thrombomodulin,TM ELISA Kit

說(shuō)明書(shū)下載

產(chǎn)品詳情

產(chǎn)品描述：

豬血栓調(diào)節(jié)蛋白(TM)酶聯(lián)免疫試劑盒（CSB-E06530p）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的TM含量。豬血栓調(diào)節(jié)蛋白（TM）是一種重要的凝血調(diào)節(jié)因子，其通過(guò)與凝血酶結(jié)合，激活蛋白C，達(dá)到抗凝和促纖溶作用?；蚬こ特i腎臟移植中的分子檢測(cè)顯示，TM的表達(dá)有助于防止血栓形成和出血并發(fā)癥，是異種移植中重要的免疫調(diào)節(jié)和抗凝因子。試劑盒檢測(cè)范圍為0.312 ng/mL-20 ng/mL，適用于實(shí)驗(yàn)室研究豬心血管疾病模型、凝血功能異常機(jī)制、炎癥相關(guān)病理過(guò)程及內(nèi)皮細(xì)胞功能評(píng)估等領(lǐng)域本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書(shū)。
別名：

N/A
縮寫(xiě)：

TM
種屬：

Sus scrofa (Pig)
樣本類(lèi)型：

serum, plasma, tissue homogenates
檢測(cè)范圍：

0.312 ng/mL-20 ng/mL
靈敏度：

0.078 ng/mL
反應(yīng)時(shí)間：

1-5h
樣本體積：

50-100ul
檢測(cè)波長(zhǎng)：

450 nm
研究領(lǐng)域：

Cardiovascular
測(cè)定原理：

quantitative
測(cè)定方法：

Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

線(xiàn)性度：

To assess the linearity of the assay, samples were spiked with high concentrations of pig TM in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	91
	Range %	86-95
1:2	Average %	97
	Range %	92-103
1:4	Average %	85
	Range %	81-87
1:8	Average %	89
	Range %	83-96

回收率：

The recovery of pig TM spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	87-96
EDTA plasma (n=4)	110	103-118

標(biāo)準(zhǔn)曲線(xiàn)：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.