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  4. Pig von Willebrand Factor,vWF ELISA Kit

Pig von Willebrand Factor,vWF ELISA Kit

  • 中文名稱：
    豬血管性血友病因子/瑞斯托霉素輔因子(VWF)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-E09395p
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥3800/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    豬血管性血友病因子/瑞斯托霉素輔因子(VWF)酶聯(lián)免疫試劑盒（CSB-E09395p）為競爭法ELISA試劑盒，定量檢測血清、血漿樣本中的VWF含量。VWF（血管性血友病因子）是一種大分子糖蛋白。在生理狀態(tài)下，它參與血小板黏附、聚集以維持正常止血功能。病理方面，其水平異常與多種疾病如血管性血友病等相關(guān)。研究常聚焦于其結(jié)構(gòu)功能關(guān)系、與疾病相互作用機(jī)制，為相關(guān)疾病診斷治療提供依據(jù)。試劑盒檢測范圍為0.625 ng/mL-40 ng/mL，該產(chǎn)品可應(yīng)用于豬凝血機(jī)制研究、出血性疾病動物模型建立、獸用止血藥物開發(fā)評估，或畜牧養(yǎng)殖中豬群凝血功能的群體健康監(jiān)測等科研場景，為探究VWF在生理病理過程中的調(diào)控機(jī)制提供可靠工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    VWF ELISA kit; F8VWFvon Willebrand factor ELISA kit; vWF ELISA kit; Fragment ELISA kit
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Sus scrofa (Pig)
  • 樣本類型：
    serum, plasma
  • 檢測范圍：
    0.625 ng/mL-40 ng/mL
  • 靈敏度：
    0.156 ng/mL
  • 反應(yīng)時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Cardiovascular
  • 測定原理：
    quantitative
  • 測定方法：
    Competitive
  • 精密度：

    Intra-assay Precision (Precision within an assay): CV%<8%

    Three samples of known concentration were tested twenty times on one plate to assess.

    Inter-assay Precision (Precision between assays): CV%<10%

    Three samples of known concentration were tested in twenty assays to assess.

  • 線性度：

    To assess the linearity of the assay, samples were spiked with high concentrations of pig vWF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

    　

    Sample

    Serum(n=4)

    1:100

    Average %

    93

    Range %

    89-99

    1:200

    Average %

    98

    Range %

    92-104

    1:400

    Average %

    89

    Range %

    82-95

    1:800

    Average %

    88

    Range %

    82-94

  • 回收率：

    The recovery of pig vWF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range

    Serum (n=5)

    97

    91-103

    EDTA plasma (n=4)

    88

    84-94

  • 標(biāo)準(zhǔn)曲線：

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

    ng/ml

    OD1

    OD2

    Average

    40

    0.084

    0.085

    0.085

    20

    0.177

    0.174

    0.176

    10

    0.365

    0.375

    0.370

    5

    0.692

    0.704

    0.698

    2.5

    1.253

    1.244

    1.249

    1.25

    1.701

    1.714

    1.708

    0.625

    2.023

    2.121

    2.072

    0

    2.323

    2.425

    2.374

  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評價

靶點詳情

  • 功能：
    Important in the maintenance of hemostasis, it promotes adhesion of platelets to the sites of vascular injury by forming a molecular bridge between sub-endothelial collagen matrix and platelet-surface receptor complex, glycoprotein Ibalpha/IX/V. Also acts as a chaperone for coagulation factor VIII, delivering it to the site of injury, stabilizing its heterodimeric structure and protecting it from premature clearance from plasma.
  • 亞細(xì)胞定位：
    Secreted. Secreted, extracellular space, extracellular matrix.
  • 組織特異性：
    Plasma.
  • 數(shù)據(jù)庫鏈接：

    STRING: 9823.ENSSSCP00000000759

    UniGene: Ssc.978