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Plant Ribulose-1,5-bisphosphate carboxylase/oxygenase(RuBisCO) ELISA Kit

  • 中文名稱:
    植物1,5-二磷酸核酮糖羧化酶/加氧酶(RuBisCO)酶聯(lián)免疫試劑盒
  • 貨號(hào):
    CSB-E16686Pl
  • 規(guī)格:
    96T/48T
  • 價(jià)格:
    ¥3600/¥2500
  • 其他:

產(chǎn)品詳情

  • 產(chǎn)品描述:
    植物1,5-二磷酸核酮糖羧化酶/加氧酶(RuBisCO)酶聯(lián)免疫試劑盒(CSB-E16686Pl)為競(jìng)爭(zhēng)法ELISA試劑盒,定量檢測(cè)植物組織樣本中的RuBisCO含量。RuBisCO即核酮糖-1,5-二磷酸羧化酶/加氧酶,是植物光合作用中催化二氧化碳固定的關(guān)鍵酶。它在全球碳循環(huán)中扮演重要角色。其研究機(jī)制主要圍繞提高它的羧化活性、降低加氧活性,以增強(qiáng)光合效率和作物產(chǎn)量。試劑盒檢測(cè)范圍為3.12 μg/mL-800 μg/mL,靈敏度為3.12 μg/mL。適用于植物生理學(xué)研究中光合作用機(jī)制解析、環(huán)境脅迫(如高溫、干旱)對(duì)酶活性影響評(píng)估、轉(zhuǎn)基因植物代謝通路改造效果驗(yàn)證等科研場(chǎng)景,為植物抗逆性改良、作物產(chǎn)量提升等研究提供可靠工具。本品僅用于科研,不用于臨床診斷,產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 縮寫:
    RuBisCO
  • 種屬:
    Plant
  • 樣本類型:
    plant tissues
  • 檢測(cè)范圍:
    3.12 μg/mL-800 μg/mL
  • 靈敏度:
    3.12 μg/mL
  • 反應(yīng)時(shí)間:
    1-5h
  • 樣本體積:
    50-100ul
  • 檢測(cè)波長:
    450 nm
  • 研究領(lǐng)域:
    Cell Biology
  • 測(cè)定原理:
    quantitative
  • 測(cè)定方法:
    Competitive
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<10%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<20%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 回收率:
    The recovery of plant RuBisCO spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    leaf tissue (n=5) 95 89-103  
  • 標(biāo)準(zhǔn)曲線:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    μg/ml OD1 OD2 Average    
    800 0.047 0.050 0.049    
    200 0.261 0.268 0.265    
    50 0.467 0.473 0.470    
    12.5 0.670 0.682 0.676    
    3.12 1.139 1.143 1.141    
    0 1.861 1.916 1.889    
  • 數(shù)據(jù)處理:
  • 貨期:
    3-5 working days

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 最新研究進(jìn)展:
    RuBisCO是一種催化光合作用的酶,在植物和一些細(xì)菌中廣泛存在。最近的研究發(fā)現(xiàn),RuBisCO還可以在大氣中吸收和固定二氧化碳,并且可以通過調(diào)節(jié)RuBisCO的活性和結(jié)構(gòu)來提高植物對(duì)氣候變化的適應(yīng)能力。