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  4. Plant hormone abscisic acid,ABA ELISA Kit

Plant hormone abscisic acid,ABA ELISA Kit

  • 中文名稱：
    植物激素脫落酸(ABA)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-E09159Pl
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥4200/￥3000
  • 其他：

產品詳情

  • 產品描述：
    植物激素脫落酸(ABA)酶聯(lián)免疫試劑盒（CSB-E09159Pl）為競爭法ELISA試劑盒，定量檢測植物組織樣本中的ABA含量。試劑盒檢測范圍為0.156 μg/ml-10 μg/ml，靈敏度為0.04 μg/mL?？删珳蕶z測植物組織樣本中ABA的含量，適用于植物生理學及分子生物學領域的科研分析；科研人員可通過該產品研究ABA在植物抗逆機制中的動態(tài)變化，解析其在果實成熟、器官脫落或環(huán)境適應中的調控作用，為農作物抗性改良及植物激素網絡研究提供可靠數據支持。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
  • 縮寫：
    ABA
  • 種屬：
    Plant
  • 樣本類型：
    plant tissues
  • 檢測范圍：
    0.156 μg/ml-10 μg/ml.
  • 靈敏度：
    0.04 μg/mL
  • 反應時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領域：
    Others
  • 測定原理：
    quantitative
  • 測定方法：
    Competitive
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<10%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<20%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 回收率：
    The recovery of plant ABA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Xylem saps from plants (n=5) 95 89-98  
    Crude extracts (n=5) 95 90-100  
                 
                 
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    μg/ml OD1 OD2 Average    
    10 0.124 0.126 0.125    
    5 0.213 0.228 0.221    
    2.5 0.357 0.361 0.359    
    1.25 0.498 0.506 0.502    
    0.625 0.686 0.701 0.694    
    0.312 1.244 1.268 1.256    
    0.156 1.510 1.534 1.522    
    0 2.241 2.297 2.269    
  • 數據處理：
  • 貨期：
    3-5 working days

引用文獻

產品評價

相關問答

■ 常見問題解答
Q:

When the samples are ground, does it matter how quickly they are processed – I have hundreds of samples and I think I will have to grind at least 96/per kit before processing a set. Is the time taken after grinding before processing further limited, just in case the sample changes its chemical composition?
My samples are wheat leaves grown under drought conditions. Some of them are small collections and I am concerned they may not reach 0.5 g as stated in the protocol. If say the sample is less than 05.g, e.g. 0.4g, does that mean I will need to use 3.6 ml of the extraction buffer, since 0.5 g to 4.5 ml is 1: 9 in ratio terms.

A:
Thanks for your inquiry. There is no strict requirement on the grind speed, but it would be better to grind the sample as faster as you can.
It is recommended to grind the sample well and then process further on testing soon.
As you mentioned that you need to prepare large QTY sample, here advice you to store the first prepared sample at 2-8 ℃。 Pls kindly note this just can be stored for one day.
If you do not make the experiment at the same time, really advice you to prepare the sample partially according to your test request.
You can check your experiment schedule and control the time for this.
In theory, the sample volume can be adapted. Generally it is recommended to take 0.5g to prepare the sample. If it is too less, the sample will not be detected.
And dear, if your sample is really not abundant, here are 2 advice: first is that pls try to collect more sample, second is that change the sample based on the ratio terms 1:9.
Q:

The freshly stored wheat leaf samples at -80 degrees Celsius will need to be freeze-dried – please state for how this needs to be. In addition, how is the grinding performed? What tools are used?

A:
Thanks for your inquiry.
Yes, the freshly stored wheat leaf samples at -80 degrees Celsius will need to be freeze-dried. You already keep them at -80 degrees Celsius, the samples are through the freeze-dried process indeed.
When do make the test, pls take 0.5g out and use the mortar usually used in lab to grind the freeze-dried samples.
And then check the detailed steps"Crude extracts " according to the manual to arrange the preliminary experiment.

靶點詳情

  • 最新研究進展：
        植物激素脫落酸（Abscisic Acid，ABA）是一種重要的植物激素，具有調節(jié)植物生長發(fā)育、應對環(huán)境脅迫等多種生理作用。近年來，對ABA的研究逐漸深入，以下是最新的研究進展：
        ABA在植物抗旱適應中的作用機制得到了深入研究。研究發(fā)現(xiàn)，ABA能夠調節(jié)植物根系的生長和分布，使根系更深入土壤中尋找水源。同時，ABA還能夠促進植物蒸騰作用的減緩，從而減少水分流失。
        ABA的代謝和信號轉導途徑研究取得了新進展。研究人員已經發(fā)現(xiàn)多種ABA受體，并且探索了它們與ABA的結合機制。同時，ABA代謝途徑中的關鍵酶類的作用機制也得到了更深入的研究。
        ABA在植物與環(huán)境互動中的作用機制也被深入探究。例如，研究發(fā)現(xiàn)ABA能夠調節(jié)植物對鹽堿脅迫的響應，同時還可以通過調節(jié)植物光合作用等途徑來增加植物對環(huán)境的適應能力。
        ABA的應用研究也取得了一定進展。ABA已經被廣泛用于植物生長調節(jié)劑和抗旱劑等領域，例如在提高作物耐旱性、改善果實品質等方面都有著廣泛的應用前景。