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  4. Rabbit Aggrecan core protein(ACAN) ELISA kit

Rabbit Aggrecan core protein(ACAN) ELISA kit

  • 中文名稱：
    兔子蛋白聚糖(ACAN)酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-E12831Rb
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥4200/￥3000
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    兔子蛋白聚糖(ACAN)酶聯(lián)免疫試劑盒（CSB-E12831Rb）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的ACAN含量。ACAN即聚集蛋白聚糖，是細(xì)胞外基質(zhì)的重要組成部分。它參與維持組織彈性和抗壓性等。研究機(jī)制方面，其異常表達(dá)與骨關(guān)節(jié)炎、腫瘤等疾病發(fā)生發(fā)展相關(guān)，如調(diào)控細(xì)胞增殖、遷移，影響微環(huán)境，目前圍繞它的干預(yù)手段是研究熱點(diǎn)。試劑盒檢測(cè)范圍為0.625 ng/mL-40 ng/mL，本試劑盒適用于軟骨代謝機(jī)制、關(guān)節(jié)疾病模型等基礎(chǔ)研究領(lǐng)域。該產(chǎn)品適用于體外評(píng)估軟骨細(xì)胞分泌功能、病理模型中的基質(zhì)代謝動(dòng)態(tài)監(jiān)測(cè)，或生物材料對(duì)軟骨再生的影響等科研場(chǎng)景。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書。
  • 別名：
    ACAN ELISA Kit; AGC1 ELISA Kit; Aggrecan core protein ELISA Kit; Cartilage-specific proteoglycan core protein ELISA Kit; CSPCP ELISA Kit; Fragment ELISA Kit
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Oryctolagus cuniculus (Rabbit)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測(cè)范圍：
    0.625 ng/mL-40 ng/mL
  • 靈敏度：
    0.156 ng/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測(cè)波長(zhǎng)：
    450 nm
  • 研究領(lǐng)域：
    Signal Transduction
  • 測(cè)定原理：
    quantitative
  • 測(cè)定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rabbit ACAN in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:1Average %98
    Range %90-103
    1:2Average %89
    Range %82-94
    1:4Average %90
    Range %82-94
    1:8Average %94
    Range %87-98
  • 回收率：
    The recovery of rabbit ACAN spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9084-94
    EDTA plasma (n=4)10393-107
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2AverageCorrected
    402.020 2.121 2.071 1.944
    201.902 1.846 1.874 1.747
    101.570 1.524 1.547 1.420
    51.238 1.200 1.219 1.092
    2.50.955 0.934 0.945 0.818
    1.250.532 0.545 0.539 0.412
    0.6250.315 0.309 0.312 0.185
    00.128 0.126 0.127
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

引用文獻(xiàn)

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    This proteoglycan is a major component of extracellular matrix of cartilagenous tissues. A major function of this protein is to resist compression in cartilage. It binds avidly to hyaluronic acid via an N-terminal globular region. May play a regulatory role in the matrix assembly of the cartilage.
  • 蛋白家族：
    Aggrecan/versican proteoglycan family
  • 數(shù)據(jù)庫(kù)鏈接：