Rabbit Bone Alkaline Phosphatase (BALP) ELISA Kit

產(chǎn)品詳情

文獻(xiàn)引用 (2) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

兔子骨堿性磷酸酶(BALP)酶聯(lián)免疫試劑盒（CSB-E15042Rb）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿樣本中的BALP含量。BALP即骨堿性磷酸酶，它是成骨細(xì)胞的表型標(biāo)志物。在骨代謝中，成骨細(xì)胞分泌BALP參與骨基質(zhì)的合成與礦化。研究通過檢測(cè)其水平來(lái)反映成骨細(xì)胞活性及骨形成狀況，在骨疾病如骨質(zhì)疏松、佝僂病等的診斷與病情監(jiān)測(cè)中有重要意義。試劑盒檢測(cè)范圍為0.156 mIU/mL-10 mIU/mL，本試劑盒適用于骨代謝相關(guān)動(dòng)物模型研究，如骨質(zhì)疏松癥機(jī)制探索、骨折愈合進(jìn)程監(jiān)測(cè)、骨再生藥物療效評(píng)估等科研場(chǎng)景，為體外定量分析提供穩(wěn)定可靠的數(shù)據(jù)支持本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
縮寫：

BALP
Uniprot No.：

G1SLU6
種屬：

Oryctolagus cuniculus (Rabbit)
樣本類型：

serum, plasma
檢測(cè)范圍：

0.156 mIU/mL-10 mIU/mL
靈敏度：

0.039 mIU/mL
反應(yīng)時(shí)間：

1-5h
樣本體積：

50-100ul
檢測(cè)波長(zhǎng)：

450 nm
研究領(lǐng)域：

Cancer
測(cè)定原理：

quantitative
測(cè)定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rabbit BALP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	97
	Range %	88-101
1:2	Average %	88
	Range %	81-92
1:4	Average %	89
	Range %	85-94
1:8	Average %	98
	Range %	90-101

回收率：

The recovery of rabbit BALP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	103	95-108
EDTA plasma (n=4)	99	91-103

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.