Rabbit Epidermal growth factor,EGF ELISA Kit
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中文名稱(chēng):兔子表皮生長(zhǎng)因子(EGF)酶聯(lián)免疫試劑盒
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貨號(hào):CSB-E06909Rb
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規(guī)格:96T/48T
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價(jià)格:¥4200/¥3000
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其他:
產(chǎn)品詳情
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產(chǎn)品描述:兔子表皮生長(zhǎng)因子(EGF)酶聯(lián)免疫試劑盒(CSB-E06909Rb)為競(jìng)爭(zhēng)法ELISA試劑盒,定量檢測(cè)血清、血漿、組織勻漿樣本中的EGF含量。表皮生長(zhǎng)因子(EGF)是一種重要的生長(zhǎng)因子,屬于表皮生長(zhǎng)因子家族,通過(guò)與其受體EGFR結(jié)合,激活細(xì)胞內(nèi)的信號(hào)傳導(dǎo)途徑,促進(jìn)細(xì)胞增殖、分化、遷移和存活。EGF在胚胎發(fā)育、組織再生和傷口愈合中發(fā)揮關(guān)鍵作用,同時(shí)其異常表達(dá)與多種疾病,尤其是癌癥的發(fā)生發(fā)展密切相關(guān)。試劑盒檢測(cè)范圍為0.5 ng/mL-8 ng/mL,特別適用于探究EGF在皮膚屏障功能、消化道黏膜修復(fù)、創(chuàng)傷愈合等研究領(lǐng)域的調(diào)控機(jī)制??蒲腥藛T可利用分析不同生理/病理?xiàng)l件下體液和組織中的EGF動(dòng)態(tài)變化,為研究生長(zhǎng)因子在再生醫(yī)學(xué)、腫瘤微環(huán)境中的作用提供量化依據(jù),其寬檢測(cè)范圍設(shè)計(jì)可有效應(yīng)對(duì)復(fù)雜生物樣本中目標(biāo)蛋白濃度的顯著波動(dòng)。本品僅用于科研,不用于臨床診斷,產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書(shū)。
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縮寫(xiě):
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Uniprot No.:
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種屬:Oryctolagus cuniculus (Rabbit)
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樣本類(lèi)型:serum, plasma, tissue homogenates
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檢測(cè)范圍:0.5 ng/mL-8 ng/mL
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靈敏度:0.25 ng/mL
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反應(yīng)時(shí)間:1-5h
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樣本體積:50-100ul
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檢測(cè)波長(zhǎng):450 nm
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研究領(lǐng)域:Others
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測(cè)定原理:quantitative
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測(cè)定方法:Competitive
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精密度:
Intra-assay Precision (Precision within an assay): CV%<15% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<15% Three samples of known concentration were tested in twenty assays to assess. -
線性度:
To assess the linearity of the assay, samples were spiked with high concentrations of rabbit EGF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:1 Average % 111 Range % 100-115 1:2 Average % 92 Range % 84-97 1:4 Average % 100 Range % 90-104 1:8 Average % 100 Range % 93-104 -
回收率:
The recovery of rabbit EGF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 94 90-98 EDTA plasma (n=4) 92 87-97 -
標(biāo)準(zhǔn)曲線:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. ng/ml OD1 OD2 Average 8 0.147 0.141 0.144 4 0.311 0.322 0.317 2 0.501 0.534 0.518 1 0.926 0.902 0.914 0.5 1.932 1.966 1.949 -
數(shù)據(jù)處理:
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貨期:3-5 working days
相關(guān)問(wèn)答
Can I check the ingredients of Stop solution for CSB-E06909Rb.
Please also advise if the standard is derived from CHO or E.coli.
Stop solution: 4N sulfuric acid
Can you please also tell me if the standard is bacurovirus derived? We don't need the sequence, just the source.
I'd like to know the necessary sample volume for this ELISA kit.
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