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  4. Rabbit Fibronectin(FN1) ELISA kit

Rabbit Fibronectin(FN1) ELISA kit

  • 中文名稱(chēng)：
    兔纖連蛋白(FN1)酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-EL008759RB
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥4200/￥3000
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    兔纖連蛋白(FN1)酶聯(lián)免疫試劑盒（CSB-EL008759RB）為競(jìng)爭(zhēng)法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的FN1含量。FN1即纖連蛋白 1，是細(xì)胞外基質(zhì)的重要成分。它參與細(xì)胞黏附、遷移、增殖等多種生理過(guò)程。在研究機(jī)制方面，F(xiàn)N1 可與多種受體相互作用，調(diào)節(jié)細(xì)胞信號(hào)通路。其異常表達(dá)與腫瘤、纖維化等疾病密切相關(guān)，是潛在治療靶點(diǎn)。試劑盒檢測(cè)范圍為1.25 ng/mL-20 ng/mL，適用于實(shí)驗(yàn)室對(duì)FN1蛋白表達(dá)水平的動(dòng)態(tài)監(jiān)測(cè)，可廣泛用于心血管疾病模型、纖維化機(jī)制研究、腫瘤微環(huán)境分析等基礎(chǔ)科研領(lǐng)域，為探索細(xì)胞外基質(zhì)調(diào)控機(jī)制提供可靠工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書(shū)。
  • 別名：
    FN1Fibronectin ELISA Kit; FN ELISA Kit; Fragment ELISA Kit
  • 縮寫(xiě)：
  • Uniprot No.：
  • 種屬：
    Oryctolagus cuniculus (Rabbit)
  • 樣本類(lèi)型：
    serum, plasma, tissue homogenates
  • 檢測(cè)范圍：
    1.25 ng/mL-20 ng/mL
  • 靈敏度：
    1.25 ng/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測(cè)波長(zhǎng)：
    450 nm
  • 研究領(lǐng)域：
    Immunology
  • 測(cè)定原理：
    quantitative
  • 測(cè)定方法：
    Competitive
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線(xiàn)性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rabbit FN1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:100Average %95
    Range %92-98
    1:200Average %103
    Range %98-108
    1:400Average %98
    Range %94-102
    1:800Average %92
    Range %87-97
  • 回收率：
    The recovery of rabbit FN1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9996-101
    EDTA plasma (n=4)8982-96
  • 標(biāo)準(zhǔn)曲線(xiàn)：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2Average
    200.150 0.160 0.155
    100.268 0.287 0.278
    50.552 0.566 0.559
    2.50.921 0.922 0.922
    1.251.477 1.424 1.451
    02.662 2.581 2.622
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    Fibronectins bind cell surfaces and various compounds including collagen, fibrin, heparin, DNA, and actin. Fibronectins are involved in cell adhesion, cell motility, opsonization, wound healing, and maintenance of cell shape. Involved in osteoblast compaction through the fibronectin fibrillogenesis cell-mediated matrix assembly process, essential for osteoblast mineralization. Participates in the regulation of type I collagen deposition by osteoblasts.
  • 亞細(xì)胞定位：
    Secreted, extracellular space, extracellular matrix.
  • 組織特異性：
    Plasma FN (soluble dimeric form) is secreted by hepatocytes. Cellular FN (dimeric or cross-linked multimeric forms), made by fibroblasts, epithelial and other cell types, is deposited as fibrils in the extracellular matrix.
  • 數(shù)據(jù)庫(kù)鏈接：