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Rabbit Growth Hormone(GH) ELISA Kit

  • 中文名稱:
    兔子生長(zhǎng)激素(GH)酶聯(lián)免疫試劑盒
  • 貨號(hào):
    CSB-E15021Rb
  • 規(guī)格:
    96T/48T
  • 價(jià)格:
    ¥3800/¥2500
  • 其他:

產(chǎn)品詳情

  • 產(chǎn)品描述:
    兔子生長(zhǎng)激素(GH)酶聯(lián)免疫試劑盒(CSB-E15021Rb)為競(jìng)爭(zhēng)法ELISA試劑盒,定量檢測(cè)血清、血漿、組織勻漿樣本中的GH1含量。GH1 基因編碼生長(zhǎng)激素,是人體生長(zhǎng)發(fā)育的關(guān)鍵調(diào)節(jié)因子。其表達(dá)異常與多種生長(zhǎng)相關(guān)疾病如巨人癥、侏儒癥等有關(guān)。研究機(jī)制主要聚焦于調(diào)控其表達(dá)的信號(hào)通路,及與其他激素協(xié)同作用機(jī)制,以探索通過(guò)干預(yù) GH1 來(lái)治療生長(zhǎng)相關(guān)疾病的方法。試劑盒檢測(cè)范圍為6.25 ng/mL-100 ng/mL,科研中常用于探究營(yíng)養(yǎng)代謝、生長(zhǎng)發(fā)育調(diào)控及內(nèi)分泌相關(guān)疾病模型的機(jī)制研究。適用于科研領(lǐng)域如動(dòng)物生理學(xué)研究、GH相關(guān)基因表達(dá)調(diào)控實(shí)驗(yàn)、藥物或營(yíng)養(yǎng)干預(yù)對(duì)激素水平影響的評(píng)估,以及兔科動(dòng)物疾病模型(如垂體功能異常)的機(jī)制探索,為實(shí)驗(yàn)室提供高效、可靠的GH定量分析工具。本品僅用于科研,不用于臨床診斷,產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書。
  • 別名:
    GH1 ELISA Kit; Somatotropin ELISA Kit; Growth hormone ELISA Kit
  • 縮寫:
  • Uniprot No.:
  • 種屬:
    Oryctolagus cuniculus (Rabbit)
  • 樣本類型:
    serum, plasma, tissue homogenates
  • 檢測(cè)范圍:
    6.25 ng/mL-100 ng/mL
  • 靈敏度:
    6.25 ng/mL
  • 反應(yīng)時(shí)間:
    1-5h
  • 樣本體積:
    50-100ul
  • 檢測(cè)波長(zhǎng):
    450 nm
  • 研究領(lǐng)域:
    Signal Transduction
  • 測(cè)定原理:
    quantitative
  • 測(cè)定方法:
    Competitive
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%        
    Three samples of known concentration were tested twenty times on one plate to assess.    
    Inter-assay Precision (Precision between assays): CV%<10%        
    Three samples of known concentration were tested in twenty assays to assess.      
                   
  • 線性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of rabbit GH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.  
      Sample Serum(n=4)    
    1:1 Average % 89    
    Range % 82-97    
    1:2 Average % 95    
    Range % 89-100    
    1:4 Average % 90    
    Range % 85-100    
    1:8 Average % 92    
    Range % 89-102    
  • 回收率:
    The recovery of rabbit GH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.  
     
    Sample Type Average % Recovery Range    
    Serum (n=5) 96 90-110    
    EDTA plasma (n=4) 95 89-100    
                   
                   
  • 標(biāo)準(zhǔn)曲線:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.  
     
    ng/ml OD1 OD2 Average      
    100 0.169 0.171 0.170      
    50 0.270 0.276 0.273      
    25 0.496 0.488 0.492      
    12.5 1.033 1.051 1.042      
    6.25 2.062 2.053 2.058      
  • 數(shù)據(jù)處理:
  • 貨期:
    3-5 working days

引用文獻(xiàn)

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能:
    Plays an important role in growth control. Its major role in stimulating body growth is to stimulate the liver and other tissues to secrete IGF-1. It stimulates both the differentiation and proliferation of myoblasts. It also stimulates amino acid uptake and protein synthesis in muscle and other tissues.
  • 亞細(xì)胞定位:
    Secreted.
  • 蛋白家族:
    Somatotropin/prolactin family
  • 數(shù)據(jù)庫(kù)鏈接: