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  4. Rabbit Insulin-like growth factor 1,IGF-1 ELISA Kit

Rabbit Insulin-like growth factor 1,IGF-1 ELISA Kit

  • 中文名稱：
    兔子胰島素樣生長(zhǎng)因子1(IGF-1)酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-E06994Rb
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥3800/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    兔子胰島素樣生長(zhǎng)因子1(IGF-1)酶聯(lián)免疫試劑盒（CSB-E06994Rb）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的IGF1含量。IGF1即胰島素樣生長(zhǎng)因子 1 ，在人體生長(zhǎng)發(fā)育、細(xì)胞增殖分化等生理過程中起重要作用。其研究機(jī)制主要聚焦于它與受體結(jié)合后激活下游信號(hào)通路，調(diào)控細(xì)胞功能。異常表達(dá)與多種疾病如腫瘤、糖尿病等相關(guān)，是藥物研發(fā)的潛在靶點(diǎn) 。試劑盒檢測(cè)范圍為0.156 ng/mL-10 ng/mL，適用于兔類動(dòng)物模型的代謝研究、生長(zhǎng)發(fā)育評(píng)估及與IGF - 1相關(guān)的疾病機(jī)制探索，如糖尿病、肌肉萎縮等病理狀態(tài)分析本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    IGF1 ELISA Kit; IGF-1Insulin-like growth factor I ELISA Kit; IGF-I ELISA Kit; Somatomedin ELISA Kit
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Oryctolagus cuniculus (Rabbit)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測(cè)范圍：
    0.156 ng/mL-10 ng/mL
  • 靈敏度：
    0.039 ng/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測(cè)波長(zhǎng)：
    450 nm
  • 研究領(lǐng)域：
    Signal Transduction
  • 測(cè)定原理：
    quantitative
  • 測(cè)定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rabbit IGF-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %98
    Range %80-100
    1:2Average %100
    Range %91-105
    1:4Average %98
    Range %92-115
    1:8Average %93
    Range %86-98
  • 回收率：
    The recovery of rabbit IGF-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9489-98
    EDTA plasma (n=4)9085-100
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2AverageCorrected
    102.715 2.725 2.720 2.605
    52.035 2.002 2.019 1.904
    2.50.989 1.078 1.034 0.919
    1.250.591 0.602 0.597 0.482
    0.6250.342 0.352 0.347 0.232
    0.3120.201 0.212 0.207 0.092
    0.1560.175 0.185 0.180 0.065
    00.111 0.119 0.115 　
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    The insulin-like growth factors, isolated from plasma, are structurally and functionally related to insulin but have a much higher growth-promoting activity. May be a physiological regulator of [1-14C]-2-deoxy-D-glucose (2DG) transport and glycogen synthesis in osteoblasts. Stimulates glucose transport in bone-derived osteoblastic (PyMS) cells and is effective at much lower concentrations than insulin, not only regarding glycogen and DNA synthesis but also with regard to enhancing glucose uptake. May play a role in synapse maturation. Ca(2+)-dependent exocytosis of IGF1 is required for sensory perception of smell in the olfactory bulb. Acts as a ligand for IGF1R. Binds to the alpha subunit of IGF1R, leading to the activation of the intrinsic tyrosine kinase activity which autophosphorylates tyrosine residues in the beta subunit thus initiatiating a cascade of down-stream signaling events leading to activation of the PI3K-AKT/PKB and the Ras-MAPK pathways. Binds to integrins ITGAV:ITGB3 and ITGA6:ITGB4. Its binding to integrins and subsequent ternary complex formation with integrins and IGFR1 are essential for IGF1 signaling. Induces the phosphorylation and activation of IGFR1, MAPK3/ERK1, MAPK1/ERK2 and AKT1.
  • 基因功能參考文獻(xiàn)：
    1. Data suggest caloric restriction modifies response of ovarian granulosa cells to leptin on cell proliferation/apoptosis, MAP kinase signaling, and release of hormones (estradiol and IGFI). The caloric restriction studies were conducted in rabbits; then, isolated rabbit granulosa cells were exposed to recombinant human leptin. (IGF1 = insulin-like growth factor I) PMID: 27894742
    2. Intraplacental gene therapy with Ad-IGF-1 corrects naturally occurring rabbit model of intrauterine growth restriction. PMID: 25738403
    3. Our studies demonstrate that the active SVCT2 is expressed in IVD cells and that the expression of this transporter is regulated by growth factors IGF-1 and dexamethasone. PMID: 23415019
    4. increased endogenous IGF1 and IGF2 expression by the blastocyst compensates for the loss of systemic insulin and IGF. PMID: 22465205
    5. IGF1 showed a significantly higher capacity to form the posterior mesoderm and primitive streak (stage 2 and 3) than blastocysts cultured without growth factors PMID: 21827825
    6. Overall, IGF-1 promoted atherosclerosis by affecting endothelial function and aging. PMID: 21219916

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  • 亞細(xì)胞定位：
    Secreted.
  • 蛋白家族：
    Insulin family
  • 數(shù)據(jù)庫(kù)鏈接：