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中文名稱:兔子白介素18(IL-18)酶聯(lián)免疫試劑盒
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貨號:CSB-E13942Rb
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規(guī)格:96T/48T
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價格:¥4200/¥3000
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其他:
產(chǎn)品詳情
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產(chǎn)品描述:兔子白介素18(IL-18)酶聯(lián)免疫試劑盒(CSB-E13942Rb)為雙抗夾心法ELISA試劑盒,定量檢測血清、血漿樣本中的IL-18含量。IL-18是一個重要靶點。它屬于白細胞介素家族,在免疫調(diào)節(jié)中發(fā)揮關(guān)鍵作用。研究發(fā)現(xiàn),IL-18參與多種炎癥相關(guān)疾病發(fā)生發(fā)展。其作用機制主要是通過結(jié)合受體激活下游信號通路,調(diào)控免疫細胞功能,為相關(guān)疾病治療提供了新方向。試劑盒檢測范圍為12.5 pg/mL-800 pg/mL,適用于科研場景中IL - 18濃度的精準分析,例如免疫機制研究、炎癥動物模型評估或疫苗開發(fā)中免疫原性評價。本品僅用于科研,不用于臨床診斷,產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
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縮寫:IL-18
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Uniprot No.:
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種屬:Oryctolagus cuniculus (Rabbit)
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樣本類型:serum, plasma
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檢測范圍:12.5 pg/mL-800 pg/mL
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靈敏度:3.12 pg/mL
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反應(yīng)時間:1-5h
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樣本體積:50-100ul
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檢測波長:450 nm
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研究領(lǐng)域:Immunology
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測定原理:quantitative
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測定方法:Sandwich
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精密度:
Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess. -
線性度:
To assess the linearity of the assay, samples were spiked with high concentrations of rabbit IL-18 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:1 Average % 91 Range % 86-95 1:2 Average % 102 Range % 97-107 1:4 Average % 91 Range % 85-97 1:8 Average % 97 Range % 91-103 -
回收率:
The recovery of rabbit IL-18 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 95 89-98 EDTA plasma (n=4) 102 92-107 -
標準曲線:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. pg/ml OD1 OD2 Average Corrected 800 2.975 2.852 2.914 2.810 400 2.088 2.076 2.082 1.978 200 1.205 1.167 1.186 1.082 100 0.570 0.590 0.580 0.476 50 0.327 0.334 0.331 0.227 25 0.213 0.208 0.211 0.107 12.5 0.145 0.140 0.143 0.039 0 0.105 0.103 0.104 -
數(shù)據(jù)處理:
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貨期:3-5 working days
引用文獻
- Pathogenesis of intradermal staphylococcal infections: rabbit experimental approach to natural Staphylococcus aureus skin infections A Mu帽oz-Silvestre,The American Journal of Pathology,2020
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