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  4. Rabbit Interleukin 6,IL-6 ELISA Kit

Rabbit Interleukin 6,IL-6 ELISA Kit

  • 中文名稱：
    兔子白介素6(IL-6)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-E06903Rb
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥3800/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    兔子白介素6(IL-6)酶聯(lián)免疫試劑盒（CSB-E06903Rb）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的IL6含量。IL6即白細(xì)胞介素6，是一種多功能細(xì)胞因子，在免疫調(diào)節(jié)、造血、炎癥反應(yīng)等生理和病理過程中發(fā)揮關(guān)鍵作用。其異常表達(dá)與多種疾病相關(guān)，如自身免疫病、癌癥。研究機制主要聚焦于其信號通路，旨在開發(fā)阻斷IL6活性的療法，以治療相關(guān)疾病。試劑盒檢測范圍為15.6 pg/mL-1000 pg/mL，適用于科研場景中炎癥機制研究、疾病動物模型評估或藥物干預(yù)效果分析本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    IL6Interleukin-6 ELISA Kit; IL-6 ELISA Kit
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Oryctolagus cuniculus (Rabbit)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    15.6 pg/mL-1000 pg/mL
  • 靈敏度：
    3.9 pg/mL
  • 反應(yīng)時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Immunology
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rabbit IL-6 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:1 Average % 88  
    Range % 80-92  
    1:2 Average % 99  
    Range % 91-105  
    1:4 Average % 101  
    Range % 92-110  
    1:8 Average % 93  
    Range % 86-98  
  • 回收率：
    The recovery of rabbit IL-6 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 93 89-98  
    EDTA plasma (n=4) 96 90-100  
                 
                 
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected  
    1000 2.840 2.785 2.813 2.710  
    500 2.316 2.223 2.270 2.167  
    250 1.580 1.567 1.574 1.471  
    125 0.935 0.874 0.905 0.802  
    62.5 0.479 0.462 0.471 0.368  
    31.2 0.266 0.254 0.260 0.157  
    15.6 0.170 0.158 0.164 0.061  
    0 0.107 0.099 0.103 　  
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

引用文獻(xiàn)

產(chǎn)品評價

平均分:
5.0分 - 3 個評價

樣品類型：血漿（抗凝劑） EDTA

樣品信息：其他 兔

稀釋比：沒有稀釋

產(chǎn)品評價： 我用CSB-E06903Rb檢測模型組術(shù)前，術(shù)中30min-2h，術(shù)后24h血漿，標(biāo)準(zhǔn)曲線用四參數(shù)模型擬合出來的R2為0.99984（詳見圖片），標(biāo)準(zhǔn)曲線符合預(yù)期且OD值在0至2.5范圍內(nèi)，且得到的數(shù)據(jù)符合預(yù)期趨勢。該試劑盒測試穩(wěn)定，后續(xù)會繼續(xù)購買96T的。

By 陳老師

樣品類型：血漿（抗凝劑） EDTA

樣品信息：其他 兔

稀釋比：沒有稀釋

產(chǎn)品評價： 第二次回購，我用CSB-E06903Rb檢測治療組術(shù)前，術(shù)中30min-2h，術(shù)后24h血漿，標(biāo)準(zhǔn)曲線用四參數(shù)模型擬合出來的R2為0.99998（詳見圖片），標(biāo)準(zhǔn)曲線符合預(yù)期且OD值在0至3.39范圍內(nèi)，且得到的數(shù)據(jù)符合預(yù)期趨勢。該試劑盒測試穩(wěn)定，治療有效

By 陳老師

樣品類型：血漿（抗凝劑） EDTA

樣品信息：其他 兔

稀釋比：沒有稀釋

產(chǎn)品評價： 我用CSB-E06903Rb測模型組術(shù)前，術(shù)中30min-2h，術(shù)后24h血漿，標(biāo)準(zhǔn)曲線用四參數(shù)模型擬合出來的R2為0.99913（詳見圖片），標(biāo)準(zhǔn)曲線做了兩列，符合預(yù)期且OD值在0至3.5范圍內(nèi)，且得到的數(shù)據(jù)符合預(yù)期趨勢。術(shù)前為150-170 pg/ml, 術(shù)中兩小時升至400 pg/ml, 術(shù)后24h降至220 pg/ml。兔子存在個體差異，標(biāo)準(zhǔn)曲線數(shù)據(jù)如圖，兔子造模的數(shù)據(jù)用FOLD CHANGE表示，準(zhǔn)備后續(xù)發(fā)論文里。

By 陳老師

靶點詳情

  • 最新研究進展：
    IL6（白細(xì)胞介素-6）是一種由多種細(xì)胞分泌的蛋白質(zhì)，參與調(diào)節(jié)免疫、發(fā)炎反應(yīng)和代謝等生理過程。近年來的研究發(fā)現(xiàn)，IL6除了作為免疫炎癥標(biāo)志物外，還參與調(diào)節(jié)體重、代謝疾病、神經(jīng)系統(tǒng)等多種疾病的發(fā)生發(fā)展。例如，有研究發(fā)現(xiàn)IL6在肥胖、糖尿病、帕金森病、阿爾茨海默病等疾病的發(fā)生中發(fā)揮了重要作用。此外，IL6還可以作為一種治療性藥物，被用于治療某些炎癥性疾病和腫瘤。
  • 功能：
    Cytokine with a wide variety of biological functions in immunity, tissue regeneration, and metabolism. Binds to IL6R, then the complex associates to the signaling subunit IL6ST/gp130 to trigger the intracellular IL6-signaling pathway. The interaction with the membrane-bound IL6R and IL6ST stimulates 'classic signaling', whereas the binding of IL6 and soluble IL6R to IL6ST stimulates 'trans-signaling'. Alternatively, 'cluster signaling' occurs when membrane-bound IL6:IL6R complexes on transmitter cells activate IL6ST receptors on neighboring receiver cells.; IL6 is a potent inducer of the acute phase response. Rapid production of IL6 contributes to host defense during infection and tissue injury, but excessive IL6 synthesis is involved in disease pathology. In the innate immune response, is synthesized by myeloid cells, such as macrophages and dendritic cells, upon recognition of pathogens through toll-like receptors (TLRs) at the site of infection or tissue injury. In the adaptive immune response, is required for the differentiation of B cells into immunoglobulin-secreting cells. Plays a major role in the differentiation of CD4(+) T cell subsets. Essential factor for the development of T follicular helper (Tfh) cells that are required for the induction of germinal-center formation. Required to drive naive CD4(+) T cells to the Th17 lineage. Also required for proliferation of myeloma cells and the survival of plasmablast cells.; Acts as an essential factor in bone homeostasis and on vessels directly or indirectly by induction of VEGF, resulting in increased angiogenesis activity and vascular permeability. Induces, through 'trans-signaling' and synergistically with IL1B and TNF, the production of VEGF. Involved in metabolic controls, is discharged into the bloodstream after muscle contraction increasing lipolysis and improving insulin resistance. 'Trans-signaling' in central nervous system also regulates energy and glucose homeostasis. Mediates, through GLP-1, crosstalk between insulin-sensitive tissues, intestinal L cells and pancreatic islets to adapt to changes in insulin demand. Also acts as a myokine. Plays a protective role during liver injury, being required for maintenance of tissue regeneration. Also has a pivotal role in iron metabolism by regulating HAMP/hepcidin expression upon inflammation or bacterial infection. Through activation of IL6ST-YAP-NOTCH pathway, induces inflammation-induced epithelial regeneration.
  • 基因功能參考文獻(xiàn)：
    1. Mild heat shock increased the production of inflammatory cytokines, IL-1beta and IL-6 in rabbit cornea cells. PMID: 23330884
  • 亞細(xì)胞定位：
    Secreted.
  • 蛋白家族：
    IL-6 superfamily
  • 數(shù)據(jù)庫鏈接：