Rabbit Leptin,LEP ELISA Kit

產(chǎn)品詳情

文獻(xiàn)引用 (2) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

兔子瘦素(LEP)酶聯(lián)免疫試劑盒（CSB-E06971Rb）為競爭法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的LEP含量。LEP即瘦素，其編碼基因是肥胖基因。它由脂肪組織分泌，可作用于下丘腦調(diào)節(jié)能量代謝和食欲。研究機(jī)制上，通過與瘦素受體結(jié)合激活信號通路，調(diào)控神經(jīng)肽表達(dá)以影響進(jìn)食行為等，在體重和代謝平衡調(diào)節(jié)中至關(guān)重要。試劑盒檢測范圍為15.6 pg/mL-1000 pg/mL，該產(chǎn)品適用于科研領(lǐng)域中對兔模型能量代謝機(jī)制、脂肪組織功能、肥胖相關(guān)疾病機(jī)制的研究，尤其適用于評估脂肪細(xì)胞分泌活性或藥物干預(yù)后LEP的動態(tài)變化。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
縮寫：

LEP
Uniprot No.：

B0KZL0
種屬：

Oryctolagus cuniculus (Rabbit)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

15.6 pg/mL-1000 pg/mL
靈敏度：

3.9 pg/mL
反應(yīng)時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Metabolism
測定原理：

quantitative
測定方法：

Competitive

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rabbit LEP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:100	Average %	96
	Range %	91-99
1:200	Average %	93
	Range %	88-97
1:400	Average %	85
	Range %	80-89
1:800	Average %	90
	Range %	84-96

回收率：

The recovery of rabbit LEP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	99	94-105
EDTA plasma (n=4)	87	83-97

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.