Rabbit Macrophage Inflammatory Protein-1α,MIP-1α ELISA kit

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產(chǎn)品詳情

產(chǎn)品描述：

兔子巨噬細(xì)胞炎性蛋白1α(MIP-1α/CCL3)酶聯(lián)免疫試劑盒（CSB-E04625Rb）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的MIP1α/CCL3含量。MIP1α/CCL3，即巨噬細(xì)胞炎癥蛋白1-a/CCL3，是CC亞家族的趨化因子，主要由單核細(xì)胞、巨噬細(xì)胞等免疫細(xì)胞產(chǎn)生。它通過結(jié)合細(xì)胞表面受體CCR1、CCR3和CCR5，誘導(dǎo)白細(xì)胞趨化，促進(jìn)T細(xì)胞進(jìn)入炎癥區(qū)域，并在肉芽腫、哮喘等自身免疫性疾病中發(fā)揮關(guān)鍵作用。試劑盒檢測范圍為7.8 pg/mL-500 pg/mL，科研人員可將其應(yīng)用于炎癥機(jī)制研究、疾病模型中的趨化因子動態(tài)監(jiān)測、免疫調(diào)節(jié)藥物篩選等方向，為探索MIP - 1α在先天免疫應(yīng)答、感染性疾病或自身免疫病中的作用提供可靠工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
別名：

MIP-1 alpha,Macrophage Inflammatory Protein-1 alpha
縮寫：

MIP1α/CCL3
種屬：

Oryctolagus cuniculus (Rabbit)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

7.8 pg/mL-500 pg/mL
靈敏度：

1.95 pg/mL
反應(yīng)時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Immunology
測定原理：

quantitative
測定方法：

Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rabbit MIP-1α in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	105
	Range %	93-109
1:2	Average %	110
	Range %	97-114
1:4	Average %	88
	Range %	83-93
1:8	Average %	95
	Range %	85-98

回收率：

The recovery of rabbit MIP-1α spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	105	95-109
EDTA plasma (n=4)	101	91-105

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.