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  4. Rabbit Myeloperoxidase (MPO) ELISA kit

Rabbit Myeloperoxidase (MPO) ELISA kit

  • 中文名稱：
    兔子髓過(guò)氧化物酶(MPO)酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-E13689Rb
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥4200/￥3000
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    兔子髓過(guò)氧化物酶(MPO)酶聯(lián)免疫試劑盒（CSB-E13689Rb）為競(jìng)爭(zhēng)法ELISA試劑盒，定量檢測(cè)血清、血漿、組織培養(yǎng)上清液、組織勻漿樣本中的MPO含量。MPO即髓過(guò)氧化物酶，是中性粒細(xì)胞和單核細(xì)胞產(chǎn)生的一種血紅素蛋白。其可催化過(guò)氧化氫和氯離子生成次氯酸，在固有免疫中發(fā)揮重要作用。異常表達(dá)的MPO會(huì)產(chǎn)生大量活性氧，與多種炎癥相關(guān)疾病和心血管疾病的發(fā)病機(jī)制有關(guān)。試劑盒檢測(cè)范圍為1.25 ng/mL-20 ng/mL，適用于基礎(chǔ)科研中炎癥機(jī)制研究、免疫相關(guān)疾病動(dòng)物模型評(píng)估、藥物干預(yù)效果分析等場(chǎng)景，例如通過(guò)檢測(cè)實(shí)驗(yàn)動(dòng)物血清或組織樣本中的MPO水平，定量評(píng)估中性粒細(xì)胞浸潤(rùn)程度及相關(guān)疾病的病理進(jìn)程，為心血管疾病、神經(jīng)退行性疾病等領(lǐng)域的機(jī)制探索提供可靠數(shù)據(jù)支持。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書(shū)。
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Oryctolagus cuniculus (Rabbit)
  • 樣本類型：
    serum, plasma, cell culture supernates, tissue homogenates
  • 檢測(cè)范圍：
    1.25 ng/mL-20 ng/mL
  • 靈敏度：
    1.25 ng/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測(cè)波長(zhǎng)：
    450 nm
  • 研究領(lǐng)域：
    Immunology
  • 測(cè)定原理：
    quantitative
  • 測(cè)定方法：
    Competitive
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<15%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<15%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rabbit MPO in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:1 Average % 96  
    Range % 91-103  
    1:2 Average % 87  
    Range % 84-90  
    1:4 Average % 95  
    Range % 89-99  
    1:8 Average % 101  
    Range % 98-105  
  • 回收率：
    The recovery of rabbit MPO spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 89 85-94  
    EDTA plasma (n=4) 93 89-97  
                 
                 
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average    
    20 0.204 0.201 0.203    
    10 0.376 0.398 0.387    
    5 0.635 0.602 0.619    
    2.5 1.134 1.122 1.128    
    1.25 2.339 2.367 2.353    
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

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