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  4. Rabbit Osteocalcin/bone gla protein,OT/BGP ELISA Kit

Rabbit Osteocalcin/bone gla protein,OT/BGP ELISA Kit

  • 中文名稱：
    兔子骨鈣素/骨谷氨酸蛋白(OT/BGP)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-E14060Rb
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥4200/￥3000
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    兔子骨鈣素/骨谷氨酸蛋白(OT/BGP)酶聯(lián)免疫試劑盒（CSB-E14060Rb）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿、細胞裂解物樣本中的BGLAP含量。BGLAP即骨鈣素，是骨組織中含量豐富的非膠原蛋白。其背景在于它是成骨細胞特異性分泌的，反映骨代謝狀態(tài)。研究機制方面，它不僅參與骨礦化和骨轉(zhuǎn)換，還在能量代謝、生殖等系統(tǒng)有調(diào)節(jié)作用，為多領(lǐng)域疾病研究提供潛在靶點。試劑盒檢測范圍為1.25 ng/mL-80 ng/mL，常用于骨代謝調(diào)控機制、骨質(zhì)疏松癥模型評估及骨骼相關(guān)藥物開發(fā)等研究。適用于體外分析骨組織工程材料對成骨細胞功能的影響、疾病模型中骨代謝動態(tài)監(jiān)測、以及骨相關(guān)生物標志物的基礎(chǔ)研究。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    BGLAP ELISA Kit; Osteocalcin ELISA Kit; Bone Gla protein ELISA Kit; BGP ELISA Kit; Gamma-carboxyglutamic acid-containing protein ELISA Kit
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Oryctolagus cuniculus (Rabbit)
  • 樣本類型：
    serum, plasma, tissue homogenates, cell lysates
  • 檢測范圍：
    1.25 ng/mL-80 ng/mL
  • 靈敏度：
    0.31 ng/mL
  • 反應時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Metabolism
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rabbit OT/BGP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %87
    Range %82-93
    1:2Average %95
    Range %89-99
    1:4Average %94
    Range %90-90
    1:8Average %94
    Range %86-98
  • 回收率：
    The recovery of rabbit OT/BGP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9290-94
    EDTA plasma (n=4)9287-96
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2AverageCorrected
    802.780 2.701 2.741 2.641
    402.124 2.056 2.090 1.990
    201.266 1.234 1.250 1.150
    100.667 0.621 0.644 0.544
    50.353 0.335 0.344 0.244
    2.50.262 0.249 0.256 0.156
    1.250.219 0.208 0.214 0.114
    00.102 0.098 0.100 　
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評價

靶點詳情

  • 功能：
    Constitutes 1-2% of the total bone protein. It binds strongly to apatite and calcium.
  • 亞細胞定位：
    Secreted.
  • 蛋白家族：
    Osteocalcin/matrix Gla protein family
  • 數(shù)據(jù)庫鏈接：