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Rabbit Sex hormone-binding globulin(SHBG) ELISA kit

  • 中文名稱:
    兔子性激素結(jié)合球蛋白(SHBG)酶聯(lián)免疫試劑盒
  • 貨號:
    CSB-EL021252RB
  • 規(guī)格:
    96T/48T
  • 價(jià)格:
    ¥4200/¥3000
  • 其他:

產(chǎn)品詳情

  • 產(chǎn)品描述:
    兔子性激素結(jié)合球蛋白(SHBG)酶聯(lián)免疫試劑盒(CSB-EL021252RB)為競爭法ELISA試劑盒,定量檢測血清、血漿、組織勻漿樣本中的SHBG含量。SHBG即性激素結(jié)合球蛋白,其可與雄激素、雌激素等結(jié)合并調(diào)節(jié)其活性。在它影響體內(nèi)性激素的分布及代謝。研究機(jī)制上,涉及探討其基因表達(dá)調(diào)控、與性激素結(jié)合的分子機(jī)制,以及在生殖、代謝等疾病中的作用機(jī)制。試劑盒檢測范圍為25 pmol/mL-400 pmol/mL,適用于評估實(shí)驗(yàn)動(dòng)物性激素代謝狀態(tài)、研究生殖系統(tǒng)疾病模型或藥物干預(yù)對激素運(yùn)輸通路的影響,為生殖生理機(jī)制、內(nèi)分泌調(diào)控及相關(guān)代謝疾病的基礎(chǔ)研究提供高效分析工具。本品僅用于科研,不用于臨床診斷,產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名:
    SHBG ELISA Kit; Sex hormone-binding globulin ELISA Kit; SHBG ELISA Kit; Sex steroid-binding protein ELISA Kit; SBP ELISA Kit; Testis-specific androgen-binding protein ELISA Kit; ABP ELISA Kit
  • 縮寫:
    SHBG
  • Uniprot No.:
  • 種屬:
    Oryctolagus cuniculus (Rabbit)
  • 樣本類型:
    serum, plasma, tissue homogenates
  • 檢測范圍:
    25 pmol/mL-400 pmol/mL
  • 靈敏度:
    25 pmol/mL
  • 反應(yīng)時(shí)間:
    1-5h
  • 樣本體積:
    50-100ul
  • 檢測波長:
    450 nm
  • 研究領(lǐng)域:
    Signal Transduction
  • 測定原理:
    quantitative
  • 測定方法:
    Competitive
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<15%        
    Three samples of known concentration were tested twenty times on one plate to assess.    
    Inter-assay Precision (Precision between assays): CV%<15%        
    Three samples of known concentration were tested in twenty assays to assess.      
                   
  • 線性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of rabbit SHBG in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.  
      Sample Serum(n=4)    
    1:1 Average % 92    
    Range % 90-103    
    1:2 Average % 89    
    Range % 83-99    
    1:4 Average % 95    
    Range % 90-101    
    1:8 Average % 89    
    Range % 85-100    
  • 回收率:
    The recovery of rabbit SHBG spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.  
     
    Sample Type Average % Recovery Range    
    Serum (n=5) 95 87-100    
    EDTA plasma (n=4) 95 89-101    
                   
                   
  • 標(biāo)準(zhǔn)曲線:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.  
     
    pmol/ml OD1 OD2 Average      
    400 0.266 0.252 0.259      
    200 0.528 0.541 0.535      
    100 0.874 0.863 0.869      
    50 1.348 1.325 1.337      
    25 2.511 2.552 2.532      
  • 數(shù)據(jù)處理:
  • 貨期:
    3-5 working days

產(chǎn)品評價(jià)

靶點(diǎn)詳情

  • 功能:
    Functions as an androgen transport protein, but may also be involved in receptor mediated processes. Each dimer binds one molecule of steroid. Specific for 5-alpha-dihydrotestosterone, testosterone, and 17-beta-estradiol. Regulates the plasma metabolic clearance rate of steroid hormones by controlling their plasma concentration.
  • 亞細(xì)胞定位:
    Secreted.
  • 數(shù)據(jù)庫鏈接: