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  4. Rabbit Soluble protein-100B,S-100B ELISA Kit

Rabbit Soluble protein-100B,S-100B ELISA Kit

  • 中文名稱(chēng)：
    兔子S100B蛋白(S-100B)酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-E06895Rb
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥4200/￥3000
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    兔子S100B蛋白(S-100B)酶聯(lián)免疫試劑盒（CSB-E06895Rb）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的S100B含量。S100B是一種神經(jīng)膠質(zhì)細(xì)胞源性鈣結(jié)合蛋白，在神經(jīng)系統(tǒng)中發(fā)揮多種作用。其與神經(jīng)發(fā)育、損傷修復(fù)等過(guò)程密切相關(guān)。研究機(jī)制主要聚焦于其參與神經(jīng)細(xì)胞內(nèi)信號(hào)傳導(dǎo)、調(diào)節(jié)神經(jīng)細(xì)胞的存活和凋亡等方面，為神經(jīng)相關(guān)疾病的研究提供了重要靶點(diǎn)。試劑盒檢測(cè)范圍為31.25 pg/mL-2000 pg/mL，支持科研人員高效分析不同樣本中S100B的濃度動(dòng)態(tài)，適用于神經(jīng)退行性疾病模型研究、腦損傷動(dòng)物實(shí)驗(yàn)、神經(jīng)膠質(zhì)瘤機(jī)制探索等基礎(chǔ)研究領(lǐng)域。為實(shí)驗(yàn)室開(kāi)展神經(jīng)生物學(xué)、細(xì)胞應(yīng)激反應(yīng)等研究提供可靠工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書(shū)。
  • 別名：
    S100B ELISA Kit; Protein S100-B ELISA Kit; S-100 protein beta chain ELISA Kit; S-100 protein subunit beta ELISA Kit; S100 calcium-binding protein B ELISA Kit
  • 縮寫(xiě)：
  • Uniprot No.：
  • 種屬：
    Oryctolagus cuniculus (Rabbit)
  • 樣本類(lèi)型：
    serum, plasma, tissue homogenates
  • 檢測(cè)范圍：
    31.25 pg/mL-2000 pg/mL
  • 靈敏度：
    7.8 pg/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測(cè)波長(zhǎng)：
    450 nm
  • 研究領(lǐng)域：
    Signal Transduction
  • 測(cè)定原理：
    quantitative
  • 測(cè)定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rabbit S-100B in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)
    1:1 Average % 88
    Range % 84-92
    1:2 Average % 94
    Range % 90-99
    1:4 Average % 103
    Range % 99-110
    1:8 Average % 93
    Range % 89-98
  • 回收率：
    The recovery of rabbit S-100B spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 95 90-100
    EDTA plasma (n=4) 97 92-104
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected
    2000 2.505 2.652 2.579 2.480
    1000 1.968 1.976 1.972 1.873
    500 1.274 1.204 1.239 1.140
    250 0.713 0.739 0.726 0.627
    125 0.436 0.424 0.430 0.331
    62.5 0.251 0.259 0.255 0.156
    31.25 0.182 0.185 0.184 0.085
    0 0.097 0.101 0.099 　
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    Weakly binds calcium but binds zinc very tightly-distinct binding sites with different affinities exist for both ions on each monomer. Physiological concentrations of potassium ion antagonize the binding of both divalent cations, especially affecting high-affinity calcium-binding sites. Binds to and initiates the activation of STK38 by releasing autoinhibitory intramolecular interactions within the kinase. Interaction with AGER after myocardial infarction may play a role in myocyte apoptosis by activating ERK1/2 and p53/TP53 signaling. Could assist ATAD3A cytoplasmic processing, preventing aggregation and favoring mitochondrial localization. May mediate calcium-dependent regulation on many physiological processes by interacting with other proteins, such as TPR-containing proteins, and modulating their activity.
  • 基因功能參考文獻(xiàn)：
    1. The time course of S100B serum values following spinal cord decompression correlates with outcome; the initial degree of paresis is not a prognostic factor to predict outcome. PMID: 21535942
  • 亞細(xì)胞定位：
    Cytoplasm. Nucleus.
  • 蛋白家族：
    S-100 family
  • 數(shù)據(jù)庫(kù)鏈接：