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Rabbit apolipoprotein B100 (Apo-B100) ELISA Kit

  • 中文名稱:
    兔載脂蛋白B100(Apo-B100)酶聯(lián)免疫試劑盒
  • 貨號(hào):
    CSB-E09805Rb
  • 規(guī)格:
    96T/48T
  • 價(jià)格:
    ¥4200/¥3000
  • 其他:

產(chǎn)品詳情

  • 產(chǎn)品描述:
    兔載脂蛋白B100(Apo-B100)酶聯(lián)免疫試劑盒(CSB-E09805Rb)為競爭法ELISA試劑盒,定量檢測血清、血漿、組織勻漿樣本中的Apo-B100含量。Apo-B100是低密度脂蛋白(LDL)的主要結(jié)構(gòu)蛋白,參與脂質(zhì)和膽固醇代謝,與動(dòng)脈粥樣硬化密切相關(guān)。研究顯示,Apo-B100通過冷凍電鏡、AlphaFold2和分子動(dòng)力學(xué)方法解析其結(jié)構(gòu),揭示其與LDL受體(LDLR)的相互作用機(jī)制,為開發(fā)針對(duì)膽固醇代謝異常的新療法提供新視角。試劑盒檢測范圍為78 ng/mL-5000 ng/mL,靈敏度為19.5 ng/mL。適用于基礎(chǔ)科研中脂代謝機(jī)制研究、動(dòng)脈粥樣硬化病理模型分析、藥物干預(yù)對(duì)脂蛋白合成影響的評(píng)估等方向,為心血管疾病相關(guān)動(dòng)物實(shí)驗(yàn)提供可靠的檢測工具本品僅用于科研,不用于臨床診斷,產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名:
    APOBApolipoprotein B ELISA kit; Fragment ELISA kit
  • 縮寫:
    Apo-B100
  • Uniprot No.:
  • 種屬:
    Oryctolagus cuniculus (Rabbit)
  • 樣本類型:
    serum, plasma, tissue homogenates
  • 檢測范圍:
    78 ng/mL-5000 ng/mL
  • 靈敏度:
    19.5 ng/mL
  • 反應(yīng)時(shí)間:
    1-5h
  • 樣本體積:
    50-100ul
  • 檢測波長:
    450 nm
  • 研究領(lǐng)域:
    Cardiovascular
  • 測定原理:
    quantitative
  • 測定方法:
    Competitive
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of rabbit Apo-B100 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)  
    1:100 Average % 96  
    Range % 90-102  
    1:200 Average % 92  
    Range % 87-96  
    1:400 Average % 104  
    Range % 98-108  
    1:800 Average % 85  
    Range % 80-89  
  • 回收率:
    The recovery of rabbit Apo-B100 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 98 93-102  
    EDTA plasma (n=4) 87 82-92  
                 
                 
  • 標(biāo)準(zhǔn)曲線:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average    
    5000 0.092 0.091 0.092    
    2500 0.146 0.138 0.142    
    1250 0.246 0.236 0.241    
    625 0.375 0.360 0.368    
    312 0.524 0.539 0.532    
    156 0.934 0.877 0.906    
    78 1.447 1.334 1.391    
    0 2.432 2.562 2.497    
  • 數(shù)據(jù)處理:
  • 貨期:
    3-5 working days

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 最新研究進(jìn)展:
        Apo-B100是由APOB基因編碼的蛋白質(zhì),是低密度脂蛋白(LDL)的主要成分之一。最新研究表明,Apo-B100在冠心病的發(fā)病中扮演了重要的角色。研究人員發(fā)現(xiàn),Apo-B100水平升高與冠心病的風(fēng)險(xiǎn)增加有關(guān)。此外,一些研究還表明,調(diào)節(jié)Apo-B100的水平可以改善血脂和血糖代謝,從而降低心血管疾病的風(fēng)險(xiǎn)。
     
  • 功能:
    Apolipoprotein B is a major protein constituent of chylomicrons (apo B-48), LDL (apo B-100) and VLDL (apo B-100). Apo B-100 functions as a recognition signal for the cellular binding and internalization of LDL particles by the apoB/E receptor.
  • 亞細(xì)胞定位:
    Cytoplasm. Secreted. Lipid droplet.
  • 組織特異性:
    Detected in liver (at protein level). Highly expressed in liver. Detected at lower levels in jejunum, duodenum and kidney.
  • 數(shù)據(jù)庫鏈接: