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  4. Rabbit basic fibroblast growth factor,bFGF ELISA Kit

Rabbit basic fibroblast growth factor,bFGF ELISA Kit

  • 中文名稱：
    兔子堿性成纖維細(xì)胞生長(zhǎng)因子(bFGF)酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-E06938Rb
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥4200/￥3000
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    兔子堿性成纖維細(xì)胞生長(zhǎng)因子(bFGF)酶聯(lián)免疫試劑盒（CSB-E06938Rb）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的FGF2含量。FGF2即成纖維細(xì)胞生長(zhǎng)因子2，在細(xì)胞的增殖、分化及血管生成等生理過(guò)程中發(fā)揮重要作用。其研究機(jī)制方面，F(xiàn)GF2通過(guò)與細(xì)胞膜上的受體結(jié)合，激活下游信號(hào)通路，影響細(xì)胞行為，在腫瘤、心血管疾病等研究領(lǐng)域受關(guān)注。試劑盒檢測(cè)范圍為7.8 pg/mL-500 pg/mL，適用于體外培養(yǎng)細(xì)胞模型、動(dòng)物組織樣本或體液樣本的bFGF含量檢測(cè)，為研究組織再生機(jī)制、腫瘤微環(huán)境調(diào)控或炎癥反應(yīng)等科研領(lǐng)域提供可靠工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書。
  • 別名：
    FGF2 ELISA Kit; Fibroblast growth factor 2 ELISA Kit; FGF-2 ELISA Kit; Basic fibroblast growth factor ELISA Kit; bFGF ELISA Kit; Heparin-binding growth factor 2 ELISA Kit; HBGF-2 ELISA Kit; Fragment ELISA Kit
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Oryctolagus cuniculus (Rabbit)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測(cè)范圍：
    7.8 pg/mL-500 pg/mL
  • 靈敏度：
    1.95 pg/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測(cè)波長(zhǎng)：
    450 nm
  • 研究領(lǐng)域：
    Cardiovascular
  • 測(cè)定原理：
    quantitative
  • 測(cè)定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rabbit bFGF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:1Average %87
    Range %84-90
    1:2Average %104
    Range %100-108
    1:4Average %85
    Range %81-89
    1:8Average %99
    Range %95-102
  • 回收率：
    The recovery of rabbit bFGF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 8380-86
    EDTA plasma (n=4)9794-100
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    5001.863 1.812 1.838 1.679
    2501.699 1.598 1.649 1.490
    1251.428 1.436 1.432 1.273
    62.51.115 1.168 1.142 0.983
    31.20.804 0.831 0.818 0.659
    15.60.563 0.574 0.569 0.410
    7.80.362 0.372 0.367 0.208
    00.159 0.158 0.159
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    Acts as a ligand for FGFR1, FGFR2, FGFR3 and FGFR4. Also acts as an integrin ligand which is required for FGF2 signaling. Binds to integrin ITGAV:ITGB3. Plays an important role in the regulation of cell survival, cell division, cell differentiation and cell migration. Functions as a potent mitogen in vitro. Can induce angiogenesis. Mediates phosphorylation of ERK1/2 and thereby promotes retinal lens fiber differentiation.
  • 亞細(xì)胞定位：
    Secreted. Nucleus.
  • 蛋白家族：
    Heparin-binding growth factors family
  • 數(shù)據(jù)庫(kù)鏈接：