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Rabbit cardiac troponin Ⅰ,cTn-ⅠELISA Kit

  • 中文名稱:
    兔心肌肌鈣蛋白Ⅰ(cTn-Ⅰ)酶聯(lián)免疫試劑盒
  • 貨號:
    CSB-E11286Rb
  • 規(guī)格:
    96T/48T
  • 價格:
    ¥4200/¥3000
  • 其他:

產(chǎn)品詳情

  • 產(chǎn)品描述:
    兔心肌肌鈣蛋白Ⅰ(cTn-Ⅰ)酶聯(lián)免疫試劑盒(CSB-E11286Rb)為雙抗夾心法ELISA試劑盒,定量檢測血清、血漿、組織勻漿樣本中的TNNI3含量。TNNI3是重要的心肌肌鈣蛋白亞基,在心肌收縮和舒張調(diào)節(jié)中發(fā)揮關(guān)鍵作用。其異常與多種心臟疾病密切相關(guān),如心肌梗死、心肌病等??蒲腥藛T通過研究其表達、結(jié)構(gòu)及功能機制,探索基于TNNI3的診斷標志物和治療靶點。試劑盒檢測范圍為47 pg/mL-3000 pg/mL,該產(chǎn)品適用于基礎(chǔ)科研中與心肌損傷相關(guān)的動物模型研究,如心肌缺血再灌注損傷、藥物心臟毒性評估或心血管疾病機制探討等領(lǐng)域。本品僅用于科研,不用于臨床診斷,產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名:
    TNNI3 ELISA Kit; Troponin I ELISA Kit; cardiac muscle ELISA Kit; Cardiac troponin I ELISA Kit
  • 縮寫:
  • Uniprot No.:
  • 種屬:
    Oryctolagus cuniculus (Rabbit)
  • 樣本類型:
    serum, plasma, tissue homogenates
  • 檢測范圍:
    47 pg/mL-3000 pg/mL
  • 靈敏度:
    11.75 pg/mL
  • 反應(yīng)時間:
    1-5h
  • 樣本體積:
    50-100ul
  • 檢測波長:
    450 nm
  • 研究領(lǐng)域:
    Others
  • 測定原理:
    quantitative
  • 測定方法:
    Sandwich
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of rabbit cTn-Ⅰ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:1Average %87
    Range %81-92
    1:2Average %96
    Range %92-99
    1:4Average %94
    Range %89-98
    1:8Average %92
    Range %88-96
  • 回收率:
    The recovery of rabbit cTn-Ⅰ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 8783-94
    EDTA plasma (n=4)8985-97
  • 標準曲線:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    30002.754 2.559 2.657 2.564
    15002.115 2.020 2.068 1.975
    7501.248 1.227 1.238 1.145
    3750.658 0.677 0.668 0.575
    187.50.403 0.412 0.408 0.315
    940.264 0.278 0.271 0.178
    470.151 0.147 0.149 0.056
    00.093 0.092 0.093
  • 數(shù)據(jù)處理:
  • 貨期:
    3-5 working days

產(chǎn)品評價

靶點詳情

  • 功能:
    Troponin I is the inhibitory subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity.
  • 蛋白家族:
    Troponin I family