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  4. Rabbit cholesteryl ester transfer protein,CETP ELISA Kit

Rabbit cholesteryl ester transfer protein,CETP ELISA Kit

  • 中文名稱(chēng)：
    兔子膽固醇酯轉(zhuǎn)移蛋白(CETP)酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-E06841Rb
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥4200/￥3000
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    兔子膽固醇酯轉(zhuǎn)移蛋白(CETP)酶聯(lián)免疫試劑盒（CSB-E06841Rb）為競(jìng)爭(zhēng)法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的CETP含量。CETP（膽固醇酯轉(zhuǎn)運(yùn)蛋白）是一種血漿糖蛋白，其功能是促進(jìn)膽固醇酯從高密度脂蛋白（HDL）轉(zhuǎn)移到低密度脂蛋白（LDL）和極低密度脂蛋白（VLDL），從而影響血脂水平。CETP抑制劑通過(guò)抑制CETP活性，增加血清HDL-C水平并降低LDL-C水平，被視為治療血脂異常的潛在方法。試劑盒檢測(cè)范圍為1.56 ng/mL-100 ng/mL，科研人員可將其應(yīng)用于高脂血癥模型兔的血清標(biāo)志物分析、脂蛋白代謝通路研究或針對(duì)CETP抑制劑的藥效學(xué)評(píng)價(jià)，可為脂質(zhì)代謝機(jī)制研究、動(dòng)物模型評(píng)估及藥物開(kāi)發(fā)中CETP活性調(diào)控分析提供可靠工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書(shū)。
  • 別名：
    CETPCholesteryl ester transfer protein ELISA kit; Lipid transfer protein I ELISA kit; Fragment ELISA kit
  • 縮寫(xiě)：
  • Uniprot No.：
  • 種屬：
    Oryctolagus cuniculus (Rabbit)
  • 樣本類(lèi)型：
    serum, plasma, tissue homogenates
  • 檢測(cè)范圍：
    1.56 ng/mL-100 ng/mL
  • 靈敏度：
    0.39 ng/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測(cè)波長(zhǎng)：
    450 nm
  • 研究領(lǐng)域：
    Metabolism
  • 測(cè)定原理：
    quantitative
  • 測(cè)定方法：
    Competitive
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線(xiàn)性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rabbit CETP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)  
    1:100 Average % 99  
    Range % 94-102  
    1:200 Average % 97  
    Range % 94-100  
    1:400 Average % 83  
    Range % 80-86  
    1:800 Average % 91  
    Range % 87-95  
  • 回收率：
    The recovery of rabbit CETP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 100 97-103  
    EDTA plasma (n=4) 84 81-89  
                 
                 
  • 標(biāo)準(zhǔn)曲線(xiàn)：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average    
    100 0.082 0.086 0.084    
    50 0.169 0.178 0.174    
    25 0.241 0.258 0.250    
    12.5 0.361 0.378 0.370    
    6.25 0.582 0.541 0.562    
    3.12 0.878 0.898 0.888    
    1.56 1.208 1.231 1.220    
    0 2.015 2.068 2.042    
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    Involved in the transfer of neutral lipids, including cholesteryl ester and triglyceride, among lipoprotein particles. Allows the net movement of cholesteryl ester from high density lipoproteins/HDL to triglyceride-rich very low density lipoproteins/VLDL, and the equimolar transport of triglyceride from VLDL to HDL. Regulates the reverse cholesterol transport, by which excess cholesterol is removed from peripheral tissues and returned to the liver for elimination.
  • 亞細(xì)胞定位：
    Secreted.
  • 蛋白家族：
    BPI/LBP/Plunc superfamily, BPI/LBP family
  • 組織特異性：
    Expressed by the liver.
  • 數(shù)據(jù)庫(kù)鏈接：