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  4. Rabbit cross linked C-telopeptide of type Ⅱ collagen (CTX-Ⅱ) ELISA Kit

Rabbit cross linked C-telopeptide of type Ⅱ collagen (CTX-Ⅱ) ELISA Kit

  • 中文名稱：
    兔子Ⅱ型膠原C端肽(CTX-Ⅱ)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-E16213Rb
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥3800/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    兔子Ⅱ型膠原C端肽(CTX-Ⅱ)酶聯(lián)免疫試劑盒（CSB-E16213Rb）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的CTX-Ⅱ含量。CTX-Ⅱ是一種由Ⅱ型膠原降解產(chǎn)生的肽段，是骨關(guān)節(jié)炎（OA）的潛在生物標(biāo)志物。其水平與關(guān)節(jié)損傷密切相關(guān)，可用于OA的早期診斷和病情評估。研究顯示，CTX-Ⅱ在OA患者關(guān)節(jié)液中水平升高，可作為疾病進展及預(yù)后的指標(biāo)。試劑盒檢測范圍為0.625 ng/mL-40 ng/mL，常用于骨關(guān)節(jié)炎、類風(fēng)濕性關(guān)節(jié)炎等疾病模型中軟骨退行性病變的機制研究。適用于科研場景中評估實驗動物軟骨代謝狀態(tài)、篩選保護軟骨組織的候選化合物，或探究病理條件下膠原降解的動態(tài)變化，可為體外實驗提供穩(wěn)定的數(shù)據(jù)支持，助力關(guān)節(jié)疾病相關(guān)的基礎(chǔ)研究與藥物開發(fā)。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    cross linked C-telopeptide of type 2 collagen (CTX-2),_x000D_ cross linked C-telopeptide of type II collagen (CTX-II)
  • 縮寫：
    CTX-Ⅱ
  • 種屬：
    Oryctolagus cuniculus (Rabbit)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    0.625 ng/mL-40 ng/mL
  • 靈敏度：
    0.156 ng/mL
  • 反應(yīng)時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Others
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rabbit CTX-Ⅱ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)  
    1:1 Average % 95  
    Range % 89-103  
    1:2 Average % 90  
    Range % 86-99  
    1:4 Average % 95  
    Range % 90-102  
    1:8 Average % 92  
    Range % 83-99  
  • 回收率：
    The recovery of rabbit CTX-Ⅱ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 97 92-103  
    EDTA plasma (n=4) 95 89-106  
                 
                 
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average Corrected  
    40 2.547 2.652 2.600 2.462  
    20 2.037 1.962 2.000 1.862  
    10 1.449 1.376 1.413 1.275  
    5 0.902 0.866 0.884 0.746  
    2.5 0.514 0.526 0.520 0.382  
    1.25 0.332 0.347 0.340 0.202  
    0.625 0.246 0.253 0.250 0.112  
    0 0.139 0.136 0.138    
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

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