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  4. Rabbit luteinizing hormone (LH) ELISA kit

Rabbit luteinizing hormone (LH) ELISA kit

  • 中文名稱：
    兔子黃體生成素（LH）酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-E17780Rb
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    兔子黃體生成素（LH）酶聯(lián)免疫試劑盒（CSB-E17780Rb）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的LH含量。LH即促黃體生成素，是腺垂體分泌的糖蛋白激素。其生理背景在于參與調(diào)節(jié)生殖系統(tǒng)，對女性排卵和男性睪酮合成起關(guān)鍵作用。研究機制上，主要通過與靶細胞上受體結(jié)合，激活信號通路，調(diào)控生殖相關(guān)基因表達與細胞功能。試劑盒檢測范圍為1.56 mIU/mL-100 mIU/mL，該產(chǎn)品可應(yīng)用于兔繁殖生物學(xué)研究、激素水平動態(tài)監(jiān)測、生殖疾病模型評估或藥物干預(yù)實驗中LH表達變化的分析，尤其適用于卵巢、垂體等組織局部LH表達水平的檢測。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    LHBLutropin subunit beta ELISA kit; Lutropin beta chain ELISA kit; Luteinizing hormone subunit beta ELISA kit; LH-B ELISA kit; LSH-B ELISA kit; LSH-beta ELISA kit
  • 縮寫：
    LH
  • Uniprot No.：
  • 種屬：
    Oryctolagus cuniculus (Rabbit)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    1.56 mIU/mL-100 mIU/mL
  • 靈敏度：
    0.39 mIU/mL
  • 反應(yīng)時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Signal Transduction
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rabbit LH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:1 Average % 96  
    Range % 90-102  
    1:2 Average % 90  
    Range % 84-95  
    1:4 Average % 96  
    Range % 89-102  
    1:8 Average % 98  
    Range % 92-104  
  • 回收率：
    The recovery of rabbit LH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 96 90-104  
    EDTA plasma (n=4) 100 94-106  
                 
                 
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    mIU/ml OD1 OD2 Average Corrected  
    100 2.817 2.839 2.828 2.670  
    50 2.220 2.281 2.251 2.093  
    25 1.525 1.579 1.552 1.394  
    12.5 0.881 0.866 0.874 0.716  
    6.25 0.557 0.569 0.563 0.405  
    3.12 0.390 0.384 0.387 0.229  
    1.56 0.252 0.261 0.257 0.099  
    0 0.157 0.159 0.158 　  
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評價

靶點詳情

  • 功能：
    Promotes spermatogenesis and ovulation by stimulating the testes and ovaries to synthesize steroids.
  • 基因功能參考文獻：
    1. This study determined the cDNA sequences of the luteinizing hormone beta-subunit (LHB) from Japanese White (JW), New Zealand White (NZW), and Dutch-Belted (Dutch) rabbits, and we compared these LHB sequences with those of other mammals. PMID: 17094992
  • 亞細胞定位：
    Secreted.
  • 蛋白家族：
    Glycoprotein hormones subunit beta family
  • 數(shù)據(jù)庫鏈接：