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  4. Rabbit matrix metalloproteinase 1,MMP-1 ELISA kit

Rabbit matrix metalloproteinase 1,MMP-1 ELISA kit

  • 中文名稱：
    兔基質(zhì)金屬蛋白酶1(MMP-1)酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-E09744Rb
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥4200/￥3000
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    兔基質(zhì)金屬蛋白酶1(MMP-1)酶聯(lián)免疫試劑盒（CSB-E09744Rb）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的MMP1含量。MMP1 即基質(zhì)金屬蛋白酶 1，在細(xì)胞外基質(zhì)降解中發(fā)揮關(guān)鍵作用。異常表達(dá)與腫瘤侵襲轉(zhuǎn)移、關(guān)節(jié)炎等疾病密切相關(guān)。其研究聚焦于解析活性調(diào)控機(jī)制和與疾病信號(hào)通路的關(guān)聯(lián)，旨在開(kāi)發(fā)針對(duì)性藥物以干預(yù)相關(guān)病理進(jìn)程。試劑盒檢測(cè)范圍為0.156 ng/mL-10 ng/mL，適用于基礎(chǔ)醫(yī)學(xué)研究領(lǐng)域，如探究心血管疾病、關(guān)節(jié)炎或皮膚纖維化等疾病模型中MMP-1的調(diào)控機(jī)制，也可用于藥物開(kāi)發(fā)過(guò)程中對(duì)MMP-1抑制劑的體外效果評(píng)估。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書(shū)。
  • 別名：
    MMP1Interstitial collagenase ELISA Kit; EC 3.4.24.7 ELISA Kit; Matrix metalloproteinase-1 ELISA Kit; MMP-1 ELISA Kit
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Oryctolagus cuniculus (Rabbit)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測(cè)范圍：
    0.156 ng/mL-10 ng/mL
  • 靈敏度：
    0.039 ng/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測(cè)波長(zhǎng)：
    450 nm
  • 研究領(lǐng)域：
    Cancer
  • 測(cè)定原理：
    quantitative
  • 測(cè)定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rabbit MMP-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:1Average %95
    Range %90-100
    1:2Average %104
    Range %97-109
    1:4Average %88
    Range %82-92
    1:8Average %98
    Range %91-102
  • 回收率：
    The recovery of rabbit MMP-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 8882-92
    EDTA plasma (n=4)10292-107
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2AverageCorrected
    102.590 2.545 2.568 2.466
    51.963 1.922 1.943 1.841
    2.51.435 1.400 1.418 1.316
    1.250.841 0.818 0.830 0.728
    0.6250.448 0.427 0.438 0.336
    0.3120.328 0.317 0.323 0.221
    0.1560.248 0.242 0.245 0.143
    00.101 0.103 0.102
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    Cleaves collagens of types I, II, and III at one site in the helical domain. Also cleaves collagens of types VII and X.
  • 基因功能參考文獻(xiàn)：
    1. These results suggest that PEP-1-SIRT2 promotes matrix metalloproteinases-induced dedifferentiation via ERK signaling in articular chondrocytes. . PMID: 27697532
    2. the total content and active forms of matrix metalloproteinases (MMPs)-1, -3, and -13 was measured to assess the potential role of the mechanical environment in regulation of matrix turnover by ligament fibroblasts. PMID: 23879595
    3. Circulating smoke components, including acrolein, contribute to vascular diseases through enhanced MMP-1 and decreased TIMP-3 secretion. PMID: 21742783
  • 亞細(xì)胞定位：
    Secreted, extracellular space, extracellular matrix.
  • 蛋白家族：
    Peptidase M10A family
  • 數(shù)據(jù)庫(kù)鏈接：